Recombinant Human PSMB5 His Protein

SDS-Page: Recombinant Human PSMB5 Protein [NBP1-50934] - 15 % SDS-PAGE (3ug)

• Reactivity: Hu
• Applications: PAGE
• Concentration: 0.25 mg/ml

Recombinant Human PSMB5 His Protein Summary

• Description: A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 60-263 of Human PSMB5
  
  

  Source: E.coli

  Amino Acid Sequence: MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSMTTT LAFKFRHGVI VAADSRATAG AYIASQTVKK VIEINPYLLG TMAGGAADCS FWERLLARQC RIYELRNKER ISVAAASKLL ANMVYQYKGM GLSMGTMICG WDKRGPGLYY VDSEGNRISG ATFSVGSGSV YAYGVMDRGY SYDLEVEQAY DLARRAIYQA TYRDAYSGGA VNLYHVREDG WIRVSSDNVA DLHEKYSGST P

• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: PSMB5
• Purity: >90%, by SDS-PAGE

Applications/Dilutions

• Dilutions:
  • SDS-Page
• Theoretical MW: 26.7 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 20 mM Tris-HCl buffer (pH 8.5), 50% glycerol, 1mM DTT, 0.15 M NaCl
• Preservative: No Preservative
• Concentration: 0.25 mg/ml
• Purity: >90%, by SDS-PAGE

Alternate Names for Recombinant Human PSMB5 His Protein

• LMPX
• LMPXproteasome beta 5 subunit
• Macropain epsilon chain
• MB1
• MB1EC 3.4.25.1
• Multicatalytic endopeptidase complex epsilon chain
• proteasome (prosome, macropain) subunit, beta type, 5
• proteasome catalytic subunit 3
• Proteasome chain 6
• Proteasome epsilon chain
• proteasome subunit beta type-5
• Proteasome subunit MB1
• Proteasome subunit X
• proteasome subunit, beta type, 5
• PSMB5
• PSX large multifunctional protease X
• XMGC104214

Background

PSMB5 is a member of the proteasome B-type family that is a 20S core beta subunit in the proteasome. This catalytic subunit is not present in the immunoproteasome and is replaced by catalytic subunit 3i (proteasome beta 8 subunit). PSMB5 has an ATP dependent proteolytic activity and involved in an ATP/ubiquitin dependent non lysosomal proteolytic pathway. Recombinant human PSMB5 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: PSMB5