PRPF8 Antibody (JG57-38)

Western Blot: PRPF8 Antibody (JG57-38) [NBP2-75630] - Analysis of PRPF8 on SiHa (1) and A549 (2) cell lysate using anti-PRPF8 antibody at 1/1,000 dilution.

Immunocytochemistry/Immunofluorescence: PRPF8 Antibody (JG57-38) [NBP2-75630] - Staining PRPF8 in SiHa cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: PRPF8 Antibody (JG57-38) [NBP2-75630] - Analysis of paraffin-embedded rat brain tissue using anti-PRPF8 antibody. Counter stained with hematoxylin.

Flow Cytometry: PRPF8 Antibody (JG57-38) [NBP2-75630] - Analysis of A549 cells with PRPF8 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.

Immunocytochemistry/Immunofluorescence: PRPF8 Antibody (JG57-38) [NBP2-75630] - Staining PRPF8 in 293T cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunocytochemistry/Immunofluorescence: PRPF8 Antibody (JG57-38) [NBP2-75630] - Staining PRPF8 in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: PRPF8 Antibody (JG57-38) [NBP2-75630] - Analysis of paraffin-embedded human colon tissue using anti-PRPF8 antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: PRPF8 Antibody (JG57-38) [NBP2-75630] - Analysis of paraffin-embedded human prostate cancer tissue using anti-PRPF8 antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: PRPF8 Antibody (JG57-38) [NBP2-75630] - Analysis of paraffin-embedded mouse testis tissue using anti-PRPF8 antibody. Counter stained with hematoxylin.

Product Discontinued

PRPF8 Antibody (JG57-38) Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Recombinant protein within Human PRPF8 aa 2,200-2,335 / 2,335. (SwissProt: Q6P2Q9 Human; SwissProt: Q99PV0 Mouse; SwissProt: G3V6H2 Rat)
• Localization: Nucleus. Spliceosome.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: PRPF8
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:50-1:100
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Western Blot 1:500-1:2000
• Theoretical MW: 274 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: TBS (pH7.4), 0.05% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for PRPF8 Antibody (JG57-38)

• apoptosis-regulated protein 1
• apoptosis-regulated protein 2
• p220
• precursor mRNA processing protein
• pre-mRNA-processing-splicing factor 8
• PRP8 homolog
• PRP8 pre-mRNA processing factor 8 homolog (S. cerevisiae)
• PRP8 pre-mRNA processing factor 8 homolog (yeast)
• Prp8
• PRP8,220 kDa U5 snRNP-specific protein
• PRPC8HPRP8
• RP13
• Splicing factor Prp8
• U5 snRNP-specific protein (220 kD), ortholog of S. cerevisiae Prp8p

Background

Pre-mRNA splicing occurs in 2 sequential transesterification steps. The protein encoded by this gene is a component of both U2- and U12-dependent spliceosomes, and found to be essential for the catalytic step II in pre-mRNA splicing process. It contains several WD repeats, which function in protein-protein interactions. This protein has a sequence similarity to yeast Prp8 protein. This gene is a candidate gene for autosomal dominant retinitis pigmentosa. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PRPF8