Protocadherin-15 Antibody

Western Blot: Protocadherin-15 Antibody [20950002] - Western Blot was performed using affinity purified SEQer PCDH15 antibody, aa(108-207) antibody. The lanes contain 5-30ug of a whole cell extract. Final concentration of antibodies = 0.1ug/ml (1:10,000 dilution). The blot was probed overnight with the SEQer PCDH15 antibody, aa(108-207) antibody. Blot was then washed according to protocol and probed with goat-anti-Rabbit-HRP conjugate at 1:5000 dilution, washed and developed using chemiluminescence (film exposure 5-30sec). The protein was detected as represented by the band shown.

ELISA: Protocadherin-15 Antibody [20950002]

Product Discontinued

Protocadherin-15 Antibody Summary

• Immunogen: This antibody is specific for the N Terminus Region of the target protein (NM_023115).
• Specificity: This product is specific for Human PCDH15.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: PCDH15
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Western Blot 1:5000-1:20000
• Theoretical MW: 216 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Human. Use in Chicken reported in scientific literature (PMID:30061390).

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 20mM Potassium Phosphate (pH 7.0) and 0.15M NaCl
• Preservative: No Preservative
• Purity: Immunogen affinity purified

Notes

Manufactured by SDIX's proprietary Genomic Antibody Technology™. GAT FAQs.

Alternate Names for Protocadherin-15 Antibody

• autosomal recessive 23
• cadherin-related family member 15
• CDHR15
• DFNB23
• DKFZp667A1711
• PCDH15
• Protocadherin15
• Protocadherin-15
• protocadherin-related 15
• USH1F

Background

PCDH15 is a calcium-dependent cell-adhesion protein. PCDH15 is essential for maintenance of normal retinal and cochlear function.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Protocadherin-15 Antibody (20950002)(3)

Publications using 20950002

• Honda A, Kita T, Seshadri SV et al. FGFR1-mediated protocadherin-15 loading mediates cargo specificity during intraflagellar transport in inner ear hair-cell kinocilia Proc. Natl. Acad. Sci. U.S.A. 2018-08-14 [PMID: 30061390] (ICC/IF, IP, WB, Chicken)
• Garrison C, Lastwika K, Zhang Y et al. Proteomic Analysis, Immune Dysregulation, and Pathway Interconnections With Obesity J Proteome Res. 2017-01-06 [PMID: 27769113] (MiAr)
  
  Details:
  Analysis is performed on plasma proteomic data to identify how obesity can alter pathways and to highlight the risk factor for disease in subjects with a high body mass index.
• Rho JH, Lampe PD. High-throughput screening for native autoantigen-autoantibody complexes using antibody microarrays J Proteome Res. 2013-05-03 [PMID: 23541305] (MiAr)
  
  Details:
  A novel method using antibody microarrays is used to detect autoantibody-antigen complexes that can potentially be useful for detection and characterization of diseases.

Bioinformatics

• Gene Symbol: PCDH15
• Entrez:
  • 65217(Human)
• Uniprot:
  • Q96QU1(Human)