Protocadherin-12 Antibody

Western blot shows lysates of bEnd.3 mouse endothelioma cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Mouse Protocadherin-12 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7926) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Protocadherin-12 at approximately 160 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

• Reactivity: Mu
• Applications: WB
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Unconjugated
• Concentration: LYOPH

Protocadherin-12 Antibody Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse Protocadherin-12
  Leu18-Ala716
  Accession # O55134
• Specificity: Detects mouse Protocadherin-12 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human PCDH-18 is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Gene: PCDH12
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Sterile PBS to a final concentration of 0.2 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Protocadherin-12 Antibody

• PCDH12
• protocadherin 12
• Protocadherin12
• Protocadherin-12
• Vascular cadherin-2
• vascular endothelial cadherin 2
• Vascular endothelial cadherin-2
• VECAD2
• VE-cad-2
• VE-cadherin 2
• VE-cadherin-2
• VE-cadherin-2protocadherin-12

Background

PCDH12 (Protocadherin 12; also VE‑Cadherin 2) is a 150‑160 kDa glycoprotein member of the PCDH family of molecules. It exhibits limited expression, being found on endothelial cells, renal mesangium, Sertoli cells, and glycogen‑positive trophoblast cells. PCDH is a transmembrane protein that forms homotypic aggregates in a calcium‑dependent manner. Although this would suggest a prominent role in cell-cell adhesion, it is unclear if this is the only activity for the molecule. In any case, it appears that PCDH12 does play an important role in placental development. Mature mouse PCDH12 is an 1163 amino acid (aa) type I transmembrane protein (aa 18‑1180). It possesses a 699 aa extracellular region (aa 18‑716) that contains six cadherin domains (aa 28‑711), plus a 21 aa transmembrane segment coupled to a lengthy 443 aa cytoplasmic domain (aa 738‑1180). Based on human, mouse PCDH12 will undergo proteolytic processing. MMP activity will first generate a 90 kDa circulating extracellular fragment and 60 kDa membrane‑bound fragment, followed by gamma ‑secretase which has the potential to further act on the membrane‑bound fragment, generating a cytosolic 50 kDa fragment. There is one potential alternative start site at Met1060. Over aa 18‑716, mouse PCDH12 shares 95% and 82% aa sequence identity with rat and human PCDH12, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PCDH12
• Uniprot:
  • O55134(Mouse)