Proprotein Convertase 2/PCSK2 Overexpression Lysate (Native)

Images

 
Western Blot: Proprotein Convertase 2 Overexpression Lysate (Adult Normal) [NBL1-14190] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Proprotein Convertase 2.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

Proprotein Convertase 2/PCSK2 Overexpression Lysate (Native) Summary

Immunogen
The lysate was created in HEK293T cells, using plasmid ID RC222498 and based on accession number NM_002594. The protein contains a C-terminal DDK tag.
Specificity
Homo sapiens proprotein convertase subtilisin/kexin type 2 (PCSK2), mRNA.
Gene
PCSK2

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
67.9 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for Proprotein Convertase 2/PCSK2

Type
Overexpression
Protein State
Native

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for Proprotein Convertase 2/PCSK2 Overexpression Lysate (Native)

  • EC 3.4.21
  • EC 3.4.21.94
  • KEX2-like endoprotease 2
  • NEC 2
  • NEC2
  • NEC2SPC2
  • neuroendocrine convertase 2
  • PC2
  • PC2NEC-2
  • PCSK2
  • Prohormone convertase 2
  • Proprotein Convertase 2
  • proprotein convertase subtilisin/kexin type 2
  • SPC2

Background

The protein encoded by this gene belongs to the subtilisin-like proprotein convertase family. The members of this family are proprotein convertases that process latent precursor proteins into their biologically active products. This encoded protein is a proinsulin-processing enzyme that plays a key role in regulating insulin biosynthesis. It is also known to cleave proopiomelanocortin, proenkephalin, prodynorphin and proluteinizing-hormone-releasing hormone. The use of alternate polyadenylation sites has been found for this gene. [provided by RefSeq]. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Publications for Proprotein Convertase 2/PCSK2 Lysate (NBL1-14190) (0)

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Bioinformatics

Gene Symbol PCSK2
Entrez