Human Prodynorphin/PDYN ELISA Kit (Colorimetric)

Human Prodynorphin/PDYN ELISA Kit (Colorimetric) [NBP3-18745] - Standard Curve Reference

• Reactivity: Hu
• Applications: ELISA
• Suitable Sample Type: Serum, plasma and tissue homogenates
• Standard Curve Range: 3.12 pg/mL - 200 pg/mL
• Sensitivity: 0.78 pg/mL

Human Prodynorphin/PDYN ELISA Kit (Colorimetric) Summary

• Description: Assay Length: 1-5 hours
• Specificity: This assay has high sensitivity and excellent specificity for detection of human Prodynorphin / PDYN. No significant cross-reactivity or interference between human Prodynorphin / PDYN and analogues was observed.
• Standard Curve Range: 3.12 pg/mL - 200 pg/mL
• Sensitivity: 0.78 pg/mL
• Assay Type: Sandwich ELISA
• Inter-Assay: CV%< 10
• Intra-Assay: CV%< 8
• Spike Recovery: 84 - 103%
• Sample Volume: 50 - 100 uL
• Kit Type: ELISA Kit (Colorimetric)
• Gene: PDYN

Applications/Dilutions

• Dilutions:
  • ELISA

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Alternate Names for Human Prodynorphin/PDYN ELISA Kit (Colorimetric)

• ADCA
• Beta-neoendorphin-dynorphin
• leu-enkephalin
• leumorphin
• neoendorphin-dynorphin-enkephalin prepropeptide
• PENKB
• preprodynorphin
• preproenkephalin B
• proenkephalin-B
• rimorphin
• SCA23

Background

The protein encoded by this gene is a preproprotein that is proteolytically processed to form the secreted opioid peptides beta-neoendorphin, dynorphin, leu-enkephalin, rimorphin, and leumorphin. These peptides are ligands for the kappa-type of opioid receptor. Dynorphin is involved in modulating responses to several psychoactive substances, including cocaine. Multiple alternatively spliced transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2010]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

FAQs for Prodynorphin/PDYN ELISA Kit (NBP3-18745). (Showing 1 - 1 of 1 FAQ).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: PDYN