PRMT1 Antibody (PRMT1-171) - BSA Free

Western Blot: PRMT1 Antibody (PRMT1-171) - BSA Free [NB120-12189] - Cell line lysates were separated on SDS-PAGE and probed with 2 ug/mL Monoclonal Anti-PRMT1 Clone: PRMT1-171. The antibody was developed using Goat Anti-Mouse IgG-Peroxidase and a chemiluminescent substrate. Lanes 1. HEK-293T 2. HeLa 3. G361 4. NRK 5. MDBK 6. MDCK 7. CHO 8. 3T3

• Reactivity: Hu, Mu, Rt, Bv, Ca, Ch, Ha, Pm
• Applications: WB, ELISA, IP
• Clone: PRMT1-171
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 2.0 mg/ml

PRMT1 Antibody (PRMT1-171) - BSA Free Summary

• Description: Novus Biologicals Mouse PRMT1 Antibody (PRMT1-171) - BSA Free (NB120-12189) is a monoclonal antibody validated for use in WB, ELISA and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Monoclonal Anti-PRMT1 is derived from the PRMT1-171 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with full length PRMT1.
• Localization: Nuclear
• Specificity: This reacts with PRMT1 and does not cross-react with PRMT2, 3, 4, and 5.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: PRMT1
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Immunoprecipitation 1:10-1:500
  • Western Blot 1:100-1:2000
• Application Notes: ELISA: Use at an assay dependent dilution. IP: Use at an assay dependent dilution. WB: Use at a concentration of 2 ug/ml (determined by using a whole cell extract of the human kidney cell line 293T). Predicted molecular weight: 42 kDa. Not tested in other applications.Optimal dilutions/concentrations should be determined by the end user.
• Theoretical MW: ~42 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Not yet tested in other species. Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 10mM PBS (pH 7.4)
• Preservative: 0.09% Sodium Azide
• Concentration: 2.0 mg/ml
• Purity: Protein A or G purified

Alternate Names for PRMT1 Antibody (PRMT1-171) - BSA Free

• ANM1
• EC 2.1.1
• EC 2.1.1.-
• EC 2.1.1.125
• EC 2.1.1.77
• HCP1
• Histone-arginine N-methyltransferase PRMT1
• HMT1 (hnRNP methyltransferase, S. cerevisiae)-like 2
• HMT1 hnRNP methyltransferase-like 2 (S. cerevisiae)
• HMT1
• HMT2
• HRMT1L2
• HRMT1L2HMT1 hnRNP methyltransferase-like 2
• Interferon receptor 1-bound protein 4
• IR1B4
• IR1B4protein arginine N-methyltransferase 1
• PRMT1
• protein arginine methyltransferase 1

Background

PRMTs are a family of proteins that either monomethylate or dimethylate the guanidino nitrogen atoms of arginine side chains. This common post-translational modification is implicated in protein trafficking, signal transduction, and transcriptional regulation. Most methyltransferases use the methyl donor, S-adenosyl-L-methionine (AdoMet), as a cofactor.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PRMT1
• Entrez:
  • 3276(Human)
  • 15469(Mouse)
  • 60421(Rat)