PRKD3/nPKC nu Antibody

Product Discontinued

PRKD3/nPKC nu Antibody Summary

• Immunogen: The immunogen recognized by this antibody maps to a region between residue 840 and the C-terminus (residue 890) of human Protein Kinase D3 (O94806).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: PRKD3
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation 2-5 ug/mg lysate
  • Western Blot 1:1000-1:5000
• Theoretical MW: 100.471 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Tris-Citrate/Phosphate (pH 7.0 - 8.0)
• Preservative: 0.09% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for PRKD3/nPKC nu Antibody

• EC 2.7.11
• EC 2.7.11.13
• EPK2
• EPK2PKD3
• nPKC nu
• nPKC-nu
• PKC nu
• PKC-NU
• PRKCN
• PRKCNnPKC-NU
• PRKD3
• Protein kinase C nu type
• protein kinase C, nu
• protein kinase D3
• Protein kinase EPK2
• protein-serine/threonine kinase
• serine/threonine-protein kinase D3

Background

Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role. The protein encoded by this gene is one of the PKC family members. This kinase can be activated rapidly by the agonists of G protein-coupled receptors. It resides in both cytoplasm and nucleus, and its nuclear accumulation is found to be dramatically enhanced in response to its activation. This kinase can also be activated after B-cell antigen receptor (BCR) engagement, which requires intact phopholipase C gamma and the involvement of other PKC family members.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for PRKD3/nPKC nu Antibody (NB100-2316)(6)

Publications using NB100-2316

• Spratley SJ, Bastea LI, Doppler H et al. Protein kinase D regulates cofilin activity through p21-activated kinase 4. J Biol Chem 2011-09-01 [PMID: 21832093]
• Azoitei N, Pusapati GV, Kleger A et al. Protein kinase D2 is a crucial regulator of tumour cell-endothelial cell communication in gastrointestinal tumours. Gut 2010-10-01 [PMID: 20732914]
• Tan M, Hao F, Xu X et al. Lysophosphatidylcholine activates a novel PKD2-mediated signaling pathway that controls monocyte migration. Arterioscler Thromb Vasc Biol 2009-09-01 [PMID: 19520973]
• Hao Q, Wang L, Tang H et al. Vascular endothelial growth factor induces protein kinase D-dependent production of proinflammatory cytokines in endothelial cells. Am J Physiol Cell Physiol 2009-04-01 [PMID: 19176759]
• Chen LA, Li J, Silva SR et al. PKD3 is the predominant protein kinase D isoform in mouse exocrine pancreas and promotes hormone-induced amylase secretion. J Biol Chem 2009-01-01 [PMID: 19028687]
• Watkins JL, Lewandowski KT, Meek SE et al. Phosphorylation of the Par-1 polarity kinase by protein kinase D regulates 14-3-3 binding and membrane association. Proc Natl Acad Sci U S A 2008-11-01 [PMID: 19011111]

FAQs for PRKD3/nPKC nu Antibody (NB100-2316). (Showing 1 - 1 of 1 FAQs).

1. I'm interested in the PRKD3 antibody #NB100-2316, specifically for its use in immunoprecipitation reactions. The Images on the data sheet for IP looks nice. Was the same antibody used for IP and Western blot detection?
   • Yes, NB100-2316 was used for both the IP and the Western blot images.

Bioinformatics

• Gene Symbol: PRKD3
• Entrez:
  • 23683(Human)
• Uniprot:
  • O94806(Human)