PRAK Antibody

Western Blot: PRAK Antibody [H00008550-D01P] - Analysis of MAPKAPK5 expression in transfected 293T cell line by MAPKAPK5 polyclonal antibody.Lane 1: MAPKAPK5 transfected lysate(54.00 KDa).Lane 2: Non-transfected lysate.

Western Blot: PRAK Antibody [H00008550-D01P] - analysis of MAPKAPK5 expression in transfected 293T cell line by MAPKAPK5 MaxPab rabbit polyclonal antibody. Lane 1: MAPKAPK5 transfected lysate (54.00 KDa). Lane 2: Non-transfected lysate.

Proximity Ligation Assay: PRAK Antibody [H00008550-D01P] - Analysis of protein-protein interactions between MAPKAPK5 and EIF4EBP1. HeLa cells were stained with anti-MAPKAPK5 rabbit purified polyclonal 1:1200 and anti-EIF4EBP1 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).

Product Discontinued

PRAK Antibody Summary

• Description: Quality control test: Antibody reactive against mammalian transfected lysate.
• Immunogen: MAPKAPK5 (NP_003659.2, 1 a.a. - 471 a.a.) full-length human protein. MSEESDMDKAIKETSILEEYSINWTQKLGAGISGPVRVCVKKSTQERFALKILLDRPKARNEVRLHMMCATHPNIVQIIEVFANSVQFPHESSPRARLLIVMEMMEGGELFHRISQHRHFTEKQASQVTKQIALALRHCHLLNIAHRDLKPENLLFKDNSLDAPVKLCDFGFAKIDQGDLMTPQFTPYYVAPQVLEAQRRHQKEKSGIIPTSPTPYTYNKSCDLWSLGVIIYVMLCGYPPFYSKHHSRTIPKDMRRKIMTGSFEFPEEEWSQISEMAKDVVRKLLKVKPEERLTIEGVLDHPWLNSTEALDNVLPSAQLMMDKAVVAGIQQAHAEQLANMRIQDLKVSLKPLHSVNNPILRKRKLLGTKPKDSVYIHDHENGAEDSNVALEKLRDVIAQCILPQAGENEDEKLNEVMQEAWKYNRECKLLRDTLQSFSWNGRGFTDKVDRLKLAEIVKQVIEEQTTSHESQ
• Specificity: MAPKAPK5 - mitogen-activated protein kinase-activated protein kinase 5,
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: MAPKAPK5
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Proximity Ligation Assay
  • Western Blot
• Application Notes: Antibody reactivity against Recombinant Protein with GST tag on ELISA and WB and also on transfected lysate in WB. GST tag alone is used as a negative control.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.4)
• Preservative: No Preservative
• Purity: Protein A purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for PRAK Antibody

• EC 2.7.11
• MAP kinase-activated protein kinase 5
• MAPK-activated protein kinase 5
• MAPKAP kinase 5
• MAPKAPK-5
• mitogen-activated protein kinase-activated protein kinase 5
• p38-regulated/activated protein kinase
• PRAKEC 2.7.11.1

Background

The protein encoded by this gene is a member of the serine/threonine kinase family. In response to cellular stress and proinflammatory cytokines, this kinase is activated through its phosphorylation by MAP kinases including MAPK1/ERK, MAPK14/p38-alpha, and MAPK11/p38-beta. In vitro, this kinase phosphorylates heat shock protein HSP27 at its physiologically relevant sites. Two alternately spliced transcript variants of this gene encoding distinct isoforms have been reported. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: MAPKAPK5
• Entrez:
  • 8550(Human)
• Uniprot:
  • NP_003659.2(Human)