Recombinant E. coli PPIL1 Protein

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SDS-Page: PPIL1 Protein [NBC1-18375] - SlyD, 21 kDa (196 aa), confirmed by MALDI-TOF with a purity of 95% by SDS - PAGE

Product Details

Summary
Reactivity EcSpecies Glossary
Applications Func, PAGE
Concentration
1 mg/ml

Order Details

Recombinant E. coli PPIL1 Protein Summary

Description
An un-tagged recombinant protein corresponding to the amino acids1-196 of E.coli PPIL1

Source: E.coli

Amino Acid Sequence: MKVAKDLVVS LAYQVRTEDG VLVDESPVSA PLDYLHGHGS LISGLETALE GHEVGDKFDV AVGANDAYGQ YDENLVQRVP KDVFMGVDEL QVGMRFLAET DQGPVPVEIT AVEDDHVVVD GNHMLAGQNL KFNVEVVAIR EATEEELAHG HVHGAHDHHH DHDHDGCCGG HGHDHGHEHG GEGCCGGKGN GGCGCH

Details of Functionality
Specific activity is > 220 nmoles/min/mg, and is defined as the amount of enzyme that cleaves 1umole of suc-AAFP-pNA per minute at 25C in Tris-Hcl pH8.0 using chymotrypsin.
Source
E. coli
Protein/Peptide Type
Biologically Active Protein
Gene
PPIL1
Purity
>95% pure by SDS-PAGE

Applications/Dilutions

Theoretical MW
21 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
20mM Tris pH 7.5
Preservative
No Preservative
Concentration
1 mg/ml
Purity
>95% pure by SDS-PAGE

Alternate Names for Recombinant E. coli PPIL1 Protein

  • CYPL1cyclophilin-related gene 1
  • EC 5.2.1.8
  • hCyPX
  • MGC678
  • peptidyl-prolyl cis-trans isomerase
  • peptidyl-prolyl cis-trans isomerase-like 1
  • peptidylprolyl isomerase (cyclophilin)-like 1
  • PPIase
  • Rotamase PPIL1

Background

SlyD is a putative folding helper protein from the Escherichia coli cytosol, which consists of an N-terminal prolyl isomerase domain of the FKBP type and a presumably unstructured C-terminal tail. It is involved in the biosynthesis of the metal cluster in the [NiFe]-hydrogenase enzymes, and exhibits several activities including that of a peptidyl-prolyl isomerase. Recombinant E.Coli slyD was expressed in E.coli and purified by conventional chromatography.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 2 years from date of receipt.

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Bioinformatics

Gene Symbol PPIL1