![Western Blot: Potassium Channel Kv3.1 Antibody (S16B-8) [NBP2-12903] - analysis of Rat brain lysates showing detection of Kv3.1 Potassium Channel protein using Mouse Anti-Kv3.1 Potassium Channel Monoclonal Antibody, Clone S16B-8 . Load: 15 ug protein. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-Kv3.1 Potassium Channel Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.](http://images.novusbio.com/fullsize/Potassium-Channel-Kv3-1-Antibody-S16B-8-Western-Blot-NBP2-12903-img0005.jpg "Western Blot: Potassium Channel Kv3.1 Antibody (S16B-8) [NBP2-12903] - analysis of Rat brain lysates showing detection of Kv3.1 Potassium Channel protein using Mouse Anti-Kv3.1 Potassium Channel Monoclonal Antibody, Clone S16B-8 . Load: 15 ug protein. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-Kv3.1 Potassium Channel Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.")
| Immunogen | Fusion protein amino acids 437-585 (C-terminus) or rat Kv3.1b |
| Localization | Membrane |
| Specificity | Detects approx 110kDa. Weakly reactive in human samples. |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | KCNC1 |
| Purity | Protein G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | 1 ug/ml of Kv3.1 Antibody was sufficient for detection of Kv3.1b in 10 ug of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary Antibody. |
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| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS (pH 7.4), 50% Glycerol |
| Preservative | 0.09% Sodium Azide |
| Concentration | 1 mg/ml |
| Purity | Protein G purified |
| Publication using NBP2-12903 | Applications | Species |
|---|---|---|
| Jin X, Xie J, Yeh CW et al. WNK1 promotes water homeostasis by acting as a central osmolality sensor for arginine vasopressin release The Journal of clinical investigation 2023-04-18 [PMID: 37071482] (KD, WB, Mouse) | KD, WB | Mouse |
Secondary Antibodies |
Isotype Controls |
Research Areas for Potassium Channel Kv3.1 Antibody (NBP2-12903)Find related products by research area.
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| Gene Symbol | KCNC1 |
![Immunohistochemistry-Paraffin: Potassium Channel Kv3.1 Antibody (S16B-8) [NBP2-12903] - Mouse brain tissue.](http://images.novusbio.com/fullsize/Potassium-Channel-Kv3-1-Antibody-S16B-8-Immunohistochemistry-Paraffin-NBP2-12903-img0004.jpg "Immunohistochemistry-Paraffin: Potassium Channel Kv3.1 Antibody (S16B-8) [NBP2-12903] - Mouse brain tissue.")
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