POMT2 Antibody - BSA Free

Immunohistochemistry-Paraffin: POMT2 Antibody [NBP1-86152] - Staining of human prostate shows strong granular cytoplasmic positivity in glandular cells.

Lane 1: Marker [kDa] 230, 110, 82, 49, 32, 26, 18Lane 2: Human cell line RT-4Lane 3: Human cell line U-251MG sp

Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells)Lane 2: NBT-II cell lysate (Rat Wistar bladder tumour cells)

• Reactivity: Hu, Mu, Rt
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

POMT2 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit POMT2 Antibody - BSA Free (NBP1-86152) is a polyclonal antibody validated for use in IHC and WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: This antibody was developed against Recombinant Protein corresponding to amino acids: GDGFFSSAFQARLSGNNLHNASIPEHLAYGSVITVKNLRMAIGYLHSHRHLYPEGIGARQQQVTTYLHKDYNNLWIIKKHNTNSDPLDPSFPVEFVRHGDIIRLEHKETSRNLHSHYHEAPMTRKHYQVTGYGINGT
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: POMT2
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin 1:50 - 1:200
  • Western Blot 0.04-0.4 ug/ml
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended.
• Control Peptide:

  POMT2 Protein (NBP1-86152PEP)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Affinity purified

Alternate Names for POMT2 Antibody - BSA Free

• DKFZp686G10254
• Dolichyl-phosphate-mannose--protein mannosyltransferase 2
• EC 2.4.1.109
• FLJ22309
• MDDGA2
• MDDGB2
• MDDGC2
• protein O-mannosyl-transferase 2
• protein-O-mannosyltransferase 2

Background

POMT2, also known as Protein-O-Mannosyltransferase 2, is an O-mannosyltransferase that must interact with POMT1 for proper enzymatic function. POMT2 is localized to the Endoplasmic Reticulum membrane, and is ubiquitously expressed at low levels, however highly expressed in the testes. Current research surrounding POMT2 has shown a connection with autosomal recessive muscular dystrophy, as well as possible interactions with Walker-Warburg syndrome, cleft palate, and malaria. POMT2 interacts with POMT1, ALG3, ALG6, ARPC3, and ATP13A1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: POMT2