PLEKHA3 Antibody (OTI5D11)

Western Blot: PLEKHA3 Antibody (5D11) [NBP2-00768] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PLEKHA3 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PLEKHA3.

Immunocytochemistry/Immunofluorescence: PLEKHA3 Antibody (5D11) [NBP2-00768] - Immunofluorescent staining of HeLa cells using anti-PLEKHA3 mouse monoclonal antibody.

Immunohistochemistry-Paraffin: PLEKHA3 Antibody (5D11) [NBP2-00768] Staining of paraffin-embedded Human lymph node tissue using anti-PLEKHA3 mouse monoclonal antibody.

Flow Cytometry: PLEKHA3 Antibody (5D11) [NBP2-00768] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-PLEKHA3 antibody, and then analyzed by flow cytometry.

Immunohistochemistry-Paraffin: PLEKHA3 Antibody (5D11) [NBP2-00768] - Staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-PLEKHA3 mouse monoclonal antibody.

Flow Cytometry: PLEKHA3 Antibody (5D11) [NBP2-00768] - Analysis of Hela cells, using anti-PLEKHA3 antibody, (Red), compared to a nonspecific negative control antibody (Blue).

Flow Cytometry: PLEKHA3 Antibody (5D11) [NBP2-00768] - Analysis of Jurkat cells, using anti-PLEKHA3 antibody, (Red), compared to a nonspecific negative control antibody (Blue).

• Reactivity: Hu, Mu, Rt
• Applications: WB, Flow, ICC/IF, IHC
• Clone: OTI5D11
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 1.1 mg/ml

PLEKHA3 Antibody (OTI5D11) Summary

• Description: Novus Biologicals Mouse PLEKHA3 Antibody (OTI5D11) (NBP2-00768) is a monoclonal antibody validated for use in IHC, WB, Flow and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Full length human recombinant protein of human PLEKHA3 (NP_061964) produced in HEK293T cell.
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: PLEKHA3
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:100
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin 1:150
  • Western Blot 1:2000
• Theoretical MW: 33.7 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 1.0% BSA and 50% Glycerol
• Preservative: 0.02% Sodium Azide
• Concentration: 1.1 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for PLEKHA3 Antibody (OTI5D11)

• FAPP-1
• FAPP1PH domain-containing family A member 3
• FLJ20067
• four-phosphate-adaptor protein 1
• Phosphatidylinositol-four-phosphate adapter protein 1
• Phosphoinositol 4-phosphate adapter protein 1
• pleckstrin homology domain containing, family A (phosphoinositide bindingspecific) member 3
• pleckstrin homology domain-containing family A member 3
• pleckstrin homology domain-containing, family A (phosphoinositide bindingspecific) member 3

Background

PLEKHA3 binds specifically to phosphatidylinositol-4-phosphate (PtdIns4P), but not to other phosphoinositides

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PLEKHA3
• Entrez:
  • 65977(Human)