Mouse PLA2G1B ELISA Kit (Colorimetric)

ELISA: Mouse PLA2G1B ELISA Kit (Colorimetric) [NBP2-80372] - Example standard curve for Mouse PLA2G1B ELISA Kit (Colorimetric). These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Product Discontinued

Mouse PLA2G1B ELISA Kit (Colorimetric) Summary

• Specificity: Recognizes both recombinant and natural Mouse PLA2G1B.
• Standard Curve Range: 2.82-180 pg/ml (example only; lot dependent)
• Sensitivity: 0.66 pg/ml
• Assay Type: Sandwich ELISA
• Inter-Assay: CV% < 6.9 %
• Intra-Assay: CV% < 3.8
• Spike Recovery: 88-91%
• Sample Volume: 100 ul
• Kit Type: ELISA Kit (Colorimetric)
• Gene: PLA2G1B

Applications/Dilutions

• Dilutions:
  • ELISA
  • Sandwich ELISA

Packaging, Storage & Formulations

• Storage: Storage is content dependent.

Alternate Names for Mouse PLA2G1B ELISA Kit (Colorimetric)

• Group IB phospholipase A2
• Phosphatidylcholine 2-acylhydrolase 1B
• phospholipase A2
• phospholipase A2, group IB (pancreas)
• PLA2
• PLA2A
• PLA2AMGC119834
• PLA2EC 3.1.1.4
• PLA2G1B
• PPLA2
• PPLA2MGC119835

Background

Phospholipase A2 (EC 3.1.1.4) catalyzes the release of fatty acids from glycero-3-phosphocholines. The best known varieties are the digestive enzymes secreted as zymogens by the pancreas of mammals. Sequences of pancreatic PLA2 enzymes from a variety of mammals have been reported. One striking feature of these enzymes is their close homology to venom phospholipases of snakes. Other forms of PLA2 have been isolated from brain, liver, lung, spleen, intestine, macrophages, leukocytes, erythrocytes, inflammatory exudates, chondrocytes, and platelets (Seilhamer et al., 1986 [PubMed 3028739]) .[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

FAQs for PLA2G1B ELISA Kit (NBP2-80372). (Showing 1 - 1 of 1 FAQ).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: PLA2G1B