Recombinant Human PKI-beta His Protein

SDS-Page: Recombinant Human PKI-beta His Protein [NBP1-51058] - 3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.

• Reactivity: Hu
• Applications: PAGE
• Concentration: 1 mg/ml

Recombinant Human PKI-beta His Protein Summary

• Description: A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-78 of Human PKI-beta
  
  

  Source: E.coli

  Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MRTDSSKMTD VESGVANFAS SARAGRRNAL PDIQSSAATD GTSDLPLKLE ALSVKEDAKE KDEKTTQDQL EKPQNEEK

• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: PKIB
• Purity: >95%, by SDS-PAGE
• Endotoxin Note: < 1.0 EU per 1 microgram of protein (determined by LAL method)

Applications/Dilutions

• Dilutions:
  • SDS-Page
• Theoretical MW: 10.6 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 20 mM Tris-HCl buffer (pH 8.0), 30% glycerol, 0.1 M NaCl
• Preservative: No Preservative
• Concentration: 1 mg/ml
• Purity: >95%, by SDS-PAGE

Alternate Names for Recombinant Human PKI-beta His Protein

• cAMP-dependent protein kinase inhibitor 2
• cAMP-dependent protein kinase inhibitor beta
• FLJ23817
• PKI-beta
• PRKACN2
• protein kinase (cAMP-dependent, catalytic) inhibitor beta

Background

PKIB, also known as cAMP-dependent protein kinase inhibitor beta, is a member of the cAMP-dependent protein kinase inhibitor family. This protein may interact with the catalytic subunit of cAMP-dependent protein kinase and act as a competitive inhibitor. At least three alternatively spliced transcript variants encoding the same protein have been reported. Recombinant human PKIB protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Publications for PKI-beta Recombinant Protein (NBP1-51058)(1)

Publication using NBP1-51058

• Averill, AM;Rehman, HT;Charles, JW;Dinh, TA;Danyal, K;Verschraegen, CF;Stein, GS;Dostmann, WR;Ramsey, JE; Inhibition of the chimeric DnaJ-PKAc enzyme by endogenous inhibitor proteins J. Cell. Biochem. 2019-04-02 [PMID: 30938854] (In vitro, Human)

Bioinformatics

• Gene Symbol: PKIB