PIWIL1/HIWI Antibody

Western Blot: PIWIL1/HIWI Antibody [H00009271-B01P] - Analysis of PIWIL1 expression in HepG2 .

Western Blot: PIWIL1/HIWI Antibody [H00009271-B01P] - analysis of PIWIL1 expression in transfected 293T cell line by PIWIL1 purified MaxPab polyclonal antibody (B01P). Lane 1: PIWIL1 transfected lysate (94.82 KDa). Lane 2: Non-transfected lysate.

Western Blot: PIWIL1/HIWI Antibody [H00009271-B01P] - Analysis of PIWIL1 expression in transfected 293T cell line by PIWIL1 polyclonal antibody. Lane 1: PIWIL1 transfected lysate(94.71 KDa). Lane 2: Non-transfected lysate.

Product Discontinued

PIWIL1/HIWI Antibody Summary

• Immunogen: PIWIL1 (NP_004755, 1 a.a. - 861 a.a.) full-length human protein. MTGRARARARGRARGQETAQLVGSTASQQPGYIQPRPQPPPAEGELFGRGRQRGTAGGTAKSQGLQISAGFQELSLAERGGRRRDFHDLGVNTRQNLDHVKESKTGSSGIIVRLSTNHFRLTSRPQWALYQYHIDYNPLMEARRLRSALLFQHEDLIGKCHAFDGTILFLPKRLQQKVTEVFSKTRNGEDVRITITLTNELPPTSPTCLQFYNIIFRRLLKIMNLQQIGRNYYNPNDPIDIPSHRLVIWPGFTTSILQYENSIMLCTDVSHKVLRSETVLDFMFNFYHQTEEHKFQEQVSKELIGLVVLTKYNNKTYRVDDIDWDQNPKSTFKKADGSEVSFLEYYRKQYNQKITDLKQPVLVSQPKRRRGPGGTLPGPAMLIPELCYLTGLTDKMRNDFNVMKDLAVHTRLTPEQRQREVGRLIDYIHKNDNVQRELRDWGLSFDSNLLSFSGRILQTEKIHQGGKTFDYNPQFADWSKETRGAPLISVKPLDNWLLIYTRRNYEAANSLIQNLFKVTPAMGMQMRKAIMIEVDDRTEAYLRVLQQKVTADTQIVVCLLSSNRKDKYDAIKKYLCTDCPTPSQCVVARTLGKQQTVMAIATKIALQMNCKMGGELWRVDIPLKLVMIVGIDCYHDMTAGRRSIAGFVASINEGMTRWFSRCIFQDRGQELVDGLKVCLQAALRAWNSCNEYMPSRIIVYRDGVGDGQLKTLVNYEVPQFLDCLKSIGRGYNPRLTVIVVKKRVNTRFFAQSGGRLQNPLPGTVIDVEVTRPEWYDFFIVSQAVRSGSVSPTHYNVIYDNSGLKPDHIQRLTYKLCHIYYNWPGVIRVPAPCQYAHKLAFLVGQSIHREPNLSLSNRLYYL
• Specificity: PIWIL1 - piwi-like 1 (Drosophila),
• Isotype: IgG
• Clonality: Polyclonal
• Host: Mouse
• Gene: PIWIL1
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Western Blot 1:500
• Application Notes: Antibody reactive against transfected lysate for western blot. It has also been used for ELISA.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.4)
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for PIWIL1/HIWI Antibody

• HIWI
• HIWIpiwi homolog
• MIWI
• piwi (Drosophila)-like 1
• PIWI
• PIWIL1
• piwi-like 1 (Drosophila)
• piwi-like protein 1

Background

This gene encodes a member of the PIWI subfamily of Argonaute proteins, evolutionarily conserved proteins containing both PAZ and Piwi motifs that play important roles in stem cell self-renewal, RNA silencing, and translational regulation in diverse organisms. The encoded protein may play a role as an intrinsic regulator of the self-renewal capacity of germline and hematopoietic stem cells. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for PIWIL1/HIWI Antibody (H00009271-B01P)(1)

Publication using H00009271-B01P

• Wang X, Sun S, Tong X et al. MiRNA-154-5p inhibits cell proliferation and metastasis by targeting PIWIL1 in glioblastoma. Brain Res 2017-08-22 [PMID: 28842123]

Bioinformatics

• Gene Symbol: PIWIL1
• Entrez:
  • 9271(Human)
• Uniprot:
  • NP_004755()