PIWIL1/HIWI Antibody (585509) [Alexa Fluor® 488]

• Reactivity: Hu
• Applications: ICC/IF
• Clone: 585509
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor 488

PIWIL1/HIWI Antibody (585509) [Alexa Fluor® 488] Summary

• Immunogen: E. coli-derived recombinant human PIWIL1/HIWI
  Arg82-Thr290
  Accession # NP_004755
• Specificity: Detects human PIWIL1/HIWI in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human (rh) PIWIL2 or rhPIWIL4 is observed.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for PIWIL1/HIWI Antibody (585509) [Alexa Fluor® 488]

• HIWI
• HIWIpiwi homolog
• MIWI
• piwi (Drosophila)-like 1
• PIWI
• PIWIL1
• piwi-like 1 (Drosophila)
• piwi-like protein 1

Background

HIWI (human homolog of drosophila piwi), also called PIWIL1 (piwi-like 1) is an 861 amino acid, 99 kDa (predicted) cytoplasmic protein of the PIWI subgroup of the Argonaute family. PIWI domains bind a class of ~29-30 nucleotide RNAs termed piRNAs (piwi domain-binding RNAs). PIWIL1 orthologs are expressed with piRNAs in germ cells and are essential for spermatogenesis in mice. CD34⁺ hematopoietic progenitor cell PIWIL1 is downregulated upon differentiation. When overexpressed in leukemic cell lines, PIWIL1 inhibits growth and induces apoptosis. Human PIWIL1 shares 95% amino acid sequence identity with mouse and rat PIWIL1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.