Phospholipase A2 XII Antibody

Images

 
Western Blot: Phospholipase A2 XII Antibody [NBP1-31693] - Sample (30 ug of whole cell lysate) A: H1299 B: Hela 12% SDS PAGE; antibody diluted at 1:1000.

Product Details

Summary
Reactivity Hu, Rt, Bv, MkSpecies Glossary
Applications WB, IHC-Fr
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.3 mg/ml

Order Details

Phospholipase A2 XII Antibody Summary

Immunogen
Carrier-protein conjugated synthetic peptide encompassing a sequence within the center region of human Phospholipase A2 XIIA. The exact sequence is proprietary.
Localization
Secreted, Cytoplasm
Predicted Species
Bovine (100%), Monkey (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
PLA2G12A
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:3000
  • Immunohistochemistry-Frozen
Application Notes
IHC-Fr usage reported in scientific literature (PMID: 24464264).
Theoretical MW
21 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publication using
NBP1-31693 in the following applications:

Reactivity Notes

Rhesus Monkey (100%). Rat reactivity reported in scientific literature (PMID: 24464264)

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
0.1M Tris (pH 7.0), 0.1M Glycine and 10% Glycerol
Preservative
0.01% Thimerosal
Concentration
0.3 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Phospholipase A2 XII Antibody

  • EC 3.1.1.4
  • group XII secreted phospholipase A2
  • group XIIA secreted phospholipase A2
  • group XIIA secretory phospholipase A2
  • GXII sPLA2
  • GXII
  • Phosphatidylcholine 2-acylhydrolase 12A
  • phospholipase A2, group XII
  • phospholipase A2, group XIIA
  • PLA2G12
  • ROSSY
  • sPLA2-XII

Background

Secreted phospholipase A2 (sPLA2) enzymes liberate arachidonic acid from phospholipids for production of eicosanoids and exert a variety of physiologic and pathologic effects. Group XII sPLA2s, such as PLA2G12A, have relatively low specific activity and are structurally and functionally distinct from other sPLA2s (Gelb et al., 2000 [PubMed 11031251]).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Phospholipase A2 XII Antibody (NBP1-31693)(1)

We have publications tested in 1 confirmed species: Rat.

We have publications tested in 2 applications: IHC-Fr, WB.


Filter By Application
IHC-Fr
(1)
WB
(1)
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Filter By Species
Rat
(1)
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Product General Protocols

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Video Protocols

WB Video Protocol

FAQs for Phospholipase A2 XII Antibody (NBP1-31693). (Showing 1 - 2 of 2 FAQ).

  1. Our customer asked about the cross reactivity of PLA2G12A antibodies. Do you have any info if these antibodies?
    • Unless specifically indicated on the datasheet they are not tested to recognize other phospholipases. The immunogen information is present for all of those so that might give some clues to the customer as to whether they might have some reactivity, but as far as direct testing is concerned on the other phospholipases this has not been performed by the lab.
  2. Our customer asked about the cross reactivity of PLA2G12A antibodies. Do you have any info if these antibodies recognize other phospholipases?
    • Unless specifically indicated on the datasheet they are not tested to recognize other phospholipases. The immunogen information is present for all of those so that might give some clues to the customer as to whether they might have some reactivity, but as far as direct testing is concerned on the other phospholipases this has not been performed by the lab.

Secondary Antibodies

 

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Bioinformatics

Gene Symbol PLA2G12A
Entrez
Uniprot