Phospholipase A2 XII Antibody (3H2C11) - BSA Free

Western Blot: Phospholipase A2 XII Antibody (3H2C11) [NBP2-37255] - Western blot analysis using PLA2G12A mAb against HEK293 (1) and PLA2G12A (AA: 21-189)-hIgGFc transfected HEK293 (2) cell lysate.

Immunohistochemistry: Phospholipase A2 XII Antibody (3H2C11) [NBP2-37255] - Immunohistochemical analysis of paraffin-embedded liver cancer tissues using PLA2G12A mouse mAb with DAB staining.

Flow Cytometry: Phospholipase A2 XII Antibody (3H2C11) [NBP2-37255] - Flow cytometric analysis of Hela cells using PLA2G12A mouse mAb (green) and negative control (red).

Western Blot: Phospholipase A2 XII Antibody (3H2C11) [NBP2-37255] - Western blot analysis using PLA2G12A mAb against human PLA2G12A (AA: 21-189) recombinant protein. (Expected MW is 44.5 kDa)

ELISA: Phospholipase A2 XII Antibody (3H2C11) [NBP2-37255] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);

• Reactivity: Hu
• Applications: WB, ELISA, Flow, IHC, CyTOF-ready
• Clone: 3H2C11
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

Phospholipase A2 XII Antibody (3H2C11) - BSA Free Summary

• Immunogen: Purified recombinant fragment of human Phospholipase A2 XII (AA: 21-189) expressed in E. Coli.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: PLA2G12A
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • ELISA 1:10000
  • Flow Cytometry 1:200 - 1:400
  • Immunohistochemistry 1:200 - 1:1000
  • Immunohistochemistry-Paraffin 1:200 - 1:1000
  • Western Blot 1:500 - 1:2000
• Application Notes: This antibody is Cytof ready.
• Theoretical MW: 21 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein G purified

Alternate Names for Phospholipase A2 XII Antibody (3H2C11) - BSA Free

• EC 3.1.1.4
• group XII secreted phospholipase A2
• group XIIA secreted phospholipase A2
• group XIIA secretory phospholipase A2
• GXII sPLA2
• GXII
• Phosphatidylcholine 2-acylhydrolase 12A
• phospholipase A2, group XII
• phospholipase A2, group XIIA
• PLA2G12
• ROSSY
• sPLA2-XII

Background

Secreted phospholipase A2 (sPLA2) enzymes liberate arachidonic acid from phospholipids for production of eicosanoids and exert a variety of physiologic and pathologic effects. Group XII sPLA2s, such as PLA2G12A, have relatively low specific activity and are structurally and functionally distinct from other sPLA2s (Gelb et al., 2000 [PubMed 11031251]).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

FAQs for Phospholipase A2 XII Antibody (NBP2-37255). (Showing 1 - 2 of 2 FAQ).

1. Our customer asked about the cross reactivity of PLA2G12A antibodies. Do you have any info if these antibodies?
   • Unless specifically indicated on the datasheet they are not tested to recognize other phospholipases. The immunogen information is present for all of those so that might give some clues to the customer as to whether they might have some reactivity, but as far as direct testing is concerned on the other phospholipases this has not been performed by the lab.
2. Our customer asked about the cross reactivity of PLA2G12A antibodies. Do you have any info if these antibodies recognize other phospholipases?
   • Unless specifically indicated on the datasheet they are not tested to recognize other phospholipases. The immunogen information is present for all of those so that might give some clues to the customer as to whether they might have some reactivity, but as far as direct testing is concerned on the other phospholipases this has not been performed by the lab.

Bioinformatics

• Gene Symbol: PLA2G12A