Phospholipase A2 XII Antibody (3H2C11)

Images

 
Western Blot: Phospholipase A2 XII Antibody (3H2C11) [NBP2-37255] - Western blot analysis using PLA2G12A mAb against HEK293 (1) and PLA2G12A (AA: 21-189)-hIgGFc transfected HEK293 (2) cell lysate.
Immunohistochemistry: Phospholipase A2 XII Antibody (3H2C11) [NBP2-37255] - Immunohistochemical analysis of paraffin-embedded liver cancer tissues using PLA2G12A mouse mAb with DAB staining.
Flow Cytometry: Phospholipase A2 XII Antibody (3H2C11) [NBP2-37255] - Flow cytometric analysis of Hela cells using PLA2G12A mouse mAb (green) and negative control (red).
Western Blot: Phospholipase A2 XII Antibody (3H2C11) [NBP2-37255] - Western blot analysis using PLA2G12A mAb against human PLA2G12A (AA: 21-189) recombinant protein. (Expected MW is 44.5 kDa)
ELISA: Phospholipase A2 XII Antibody (3H2C11) [NBP2-37255] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, Flow, IHC, IHC-P, CyTOF-ready
Clone
3H2C11
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

Phospholipase A2 XII Antibody (3H2C11) Summary

Immunogen
Purified recombinant fragment of human PLA2G12A (AA: 21-189) expressed in E. Coli.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
PLA2G12A
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500 - 1:2000
  • ELISA 1:10000
  • Flow Cytometry 1:200 - 1:400
  • Immunohistochemistry 1:200 - 1:1000
  • Immunohistochemistry-Paraffin 1:200 - 1:1000
  • CyTOF-ready
Application Notes
This antibody is Cytof ready.
Theoretical MW
21 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
Phospholipase A2 XII Knockout HeLa Cell Lysate

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.05% Sodium Azide
Purity
Protein A or G purified

Alternate Names for Phospholipase A2 XII Antibody (3H2C11)

  • EC 3.1.1.4
  • group XII secreted phospholipase A2
  • group XIIA secreted phospholipase A2
  • group XIIA secretory phospholipase A2
  • GXII sPLA2
  • GXII
  • Phosphatidylcholine 2-acylhydrolase 12A
  • phospholipase A2, group XII
  • phospholipase A2, group XIIA
  • PLA2G12
  • ROSSY
  • sPLA2-XII

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol

FAQs for Phospholipase A2 XII Antibody (NBP2-37255). (Showing 1 - 2 of 2 FAQ).

  1. Our customer asked about the cross reactivity of PLA2G12A antibodies. Do you have any info if these antibodies?
    • Unless specifically indicated on the datasheet they are not tested to recognize other phospholipases. The immunogen information is present for all of those so that might give some clues to the customer as to whether they might have some reactivity, but as far as direct testing is concerned on the other phospholipases this has not been performed by the lab.
  2. Our customer asked about the cross reactivity of PLA2G12A antibodies. Do you have any info if these antibodies recognize other phospholipases?
    • Unless specifically indicated on the datasheet they are not tested to recognize other phospholipases. The immunogen information is present for all of those so that might give some clues to the customer as to whether they might have some reactivity, but as far as direct testing is concerned on the other phospholipases this has not been performed by the lab.

Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol PLA2G12A