Pescadillo Antibody

Western Blot: Pescadillo Antibody [NB100-1258] - Hela lysate.

Immunohistochemistry-Paraffin: Pescadillo Antibody [NB100-1258] - Human Testis.

• Reactivity: Hu, Mu, Po, Bv
• Applications: WB, ELISA, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

Pescadillo Antibody Summary

• Description: Novus Biologicals Goat Pescadillo Antibody (NB100-1258) is a polyclonal antibody validated for use in IHC, WB and ELISA. Anti-Pescadillo Antibody: Cited in 2 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Peptide with sequence GGLEKKKYERGSA-C corresponding to N-Terminus according to NP_055118.1.
• Localization: Nuclear
• Predicted Species: Mouse (100%), Porcine (100%), Bovine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: PES1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 2.5 - 3.8 ug/ml
  • Immunohistochemistry-Paraffin 2.5 - 3.8 ug/ml
  • Peptide ELISA Detection limit 1:32000
  • Western Blot 1 - 3 ug/ml
• Application Notes: WB: Approx. 70 kDa band observed in HeLa lysate (calculated MW of 68 kDa band according to NP_055118). IHC-P: Human testis shows nucleolar staining in spermatocytes and spermatids.
• Control:

  HeLa Whole Cell Lysate

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Pescadillo Antibody

• pescadillo homolog 1, containing BRCT domain (zebrafish)
• pescadillo homolog
• PESpescadillo (zebrafish) homolog 1, containing BRCT domain

Background

Pescadillo may play a crucial role in cell proliferation and may be necessary for oncogenic transformation and tumor progression.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Pescadillo Antibody (NB100-1258)(2)

Publications using NB100-1258

• Sikorski EM, Uo T, Morrison RS et al. Pescadillo interacts with the cadmium response element of the human heme oxygenase-1 promoter in renal epithelial cells. J Biol Chem 2006-08-01 [PMID: 16816389]
• Allende ML, Amsterdam A, Becker T et al. Insertional mutagenesis in zebrafish identifies two novel genes, pescadillo and dead eye, essential for embryonic development. Genes Dev 1996-12-15 [PMID: 8985183]

Bioinformatics

• Gene Symbol: PES1
• Entrez:
  • 23481(Human)
  • 64934(Mouse)
• Uniprot:
  • NP_055118.(Human)