PDE5A Antibody

Western Blot: PDE5A Antibody [NBP3-12207] - WB recombinant NBP3-12207 antibody. Overexpressing cells were treated with or without ANP. 1:500 in antibody dilution in DilObuffer. Blot was stripped with StripObuffer.

• Reactivity: Hu, Mu, Rt, Po, Bv, Sh
• Applications: WB, DB, ELISA, IHC, IP, ICC/IF
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

PDE5A Antibody Summary

• Immunogen: Phosphorylated synthetic peptide common to all PDE5A variants taken within amino acid region 120-170 on human PDE5A protein.
• Clonality: Polyclonal
• Host: Rabbit
• Gene: PDE5A
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Dot Blot 1:10000
  • ELISA 1:10000
  • Immunocytochemistry 1:100
  • Immunofluorescence 1:100
  • Immunohistochemistry 1:100
  • Immunoprecipitation 1:200
  • Western Blot 1:500

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Contains Tris, HCl/glycine buffer (pH 7.4-7.8), 30% glycerol, 0.5% BSA, along with cryo-protective agents, and HEPES (exact storage and stabilization buffer information is proprietary).
• Preservative: 0.02% Sodium Azide
• Purity: Affinity purified

Alternate Names for PDE5A Antibody

• CGB-PDE
• cGMP-binding cGMP-specific 3'-5'-cyclic nucleotide phosphodiesterase
• cGMP-binding cGMP-specific phosphodiesterase
• cGMP-specific 3'-5'-cyclic phosphodiesterase
• cGMP-specific phosphodiesterase type 5A
• CN5A
• EC 3.1.4
• EC 3.1.4.35
• PDE5
• PDE5A
• phosphodiesterase 5A, cGMP-specific
• phosphodiesterase isozyme 5

Background

PDE5A encodes a cGMP-binding, cGMP-specific phosphodiesterase, a member of the cyclic nucleotide phosphodiesterase family. This phosphodiesterase specifically hydrolyzes cGMP to 5'-GMP. It is involved in the regulation of intracellular concentrations of cyclic nucleotides and is important for smooth muscle relaxation in the cardiovascular system. Alternative splicing of this gene results in three transcript variants encoding distinct isoforms.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PDE5A