P4HA3 Overexpression Lysate (Native)

Images

 
Western Blot: P4HA3 Overexpression Lysate (Adult Normal) [NBL1-14053] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for P4HA3.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

P4HA3 Overexpression Lysate (Native) Summary

Immunogen
The lysate was created in HEK293T cells, using plasmid ID RC224487 and based on accession number NM_182904. The protein contains a C-terminal DDK tag.
Specificity
Homo sapiens prolyl 4-hydroxylase, alpha polypeptide III (P4HA3), mRNA.
Gene
P4HA3

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
59.3 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for P4HA3

Type
Overexpression
Protein State
Native

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for P4HA3 Overexpression Lysate (Native)

  • 2-oxoglutarate-4-dioxygenase subunit alpha-3
  • 4-PH alpha-3
  • alpha polypeptide III
  • collagen prolyl 4-hydroxylase alpha(III)
  • C-P4H alpha III
  • C-P4Halpha(III)
  • EC 1.14.11.2
  • Procollagen-proline
  • procollagen-proline, 2-oxoglutarate 4-dioxygenase (proline 4-hydroxylase)
  • prolyl 4-hydroxylase subunit alpha-3
  • prolyl 4-hydroxylase, alpha polypeptide III

Background

This gene encodes a component of prolyl 4-hydroxylase, a key enzyme in collagen synthesis composed of two identical alpha subunits and two beta subunits. The encoded protein is one of several different types of alpha subunits and provides the major part of the catalytic site of the active enzyme. In collagen and related proteins, prolyl 4-hydroxylase catalyzes the formation of 4-hydroxyproline that is essential to the proper three-dimensional folding of newly synthesized procollagen chains. Alternatively spliced transcript variants have been observed, but their full-length nature has not been determined. [provided by RefSeq].

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Bioinformatics

Gene Symbol P4HA3