P2X3/P2RX3 Antibody

Immunocytochemistry/Immunofluorescence: P2X3/P2RX3 Antibody [NB100-1654] - Double immunofluorescence staining in cultured human bladder urothelial cells (BUCs). BUCs show cytokeratin (red areas) and P2X3 (green areas) expression.

Immunohistochemistry-Paraffin: P2X3/P2RX3 Antibody [NB100-1654] - DRG sections double-labeled for ASIC3 (red) and either P2X3 (green, A) or TRPV1 (green, B); double-labeled cells are yellow or orange

Flow Cytometry: P2X3/P2RX3 Antibody [NB100-1654] - Mouse P2X3 is detected as seen from the shift in PE-A from stain control to P2X3 stained mast cells.Bone marrow derived mouse mast cells were fixed and permeabilized in 4% PFA/saponin and stained with this product at 4C and a secondary antibody, PE anti-rabbit.

Product Discontinued

P2X3/P2RX3 Antibody Summary

• Immunogen: Synthetic peptide: VEKQSTDSGAYSIGH, corresponding to amino acids 383-397 of C-terminus of Rat P2X3.
• Localization: Cell Membrane
• Specificity: P2X3
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: P2RX3
• Purity: Unpurified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:10-1:1000
  • Immunocytochemistry/ Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Immunoprecipitation
  • Western Blot 1:100-1:2000
• Application Notes: IP reactivity reported in (PMID: 21999289)
• Control:

  P2X3/P2RX3 Overexpression Lysate

Reactivity Notes

Mouse reactivity reported in (PMID: 27893742), Customer review.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: Whole antisera
• Preservative: 0.05% Sodium Azide
• Purity: Unpurified

Alternate Names for P2X3/P2RX3 Antibody

• ATP receptor
• MGC129956
• P2RX3
• P2X purinoceptor 3
• P2X receptor, subunit 3
• P2X3
• P2X3purinergic receptor P2X3
• purinergic receptor P2X, ligand-gated ion channel, 3
• Purinergic receptor
• purinoceptor P2X3

Background

P2RX3 is a member of the family of purinoceptors for ATP. This receptor functions as a ligand-gated ion channel and transduces ATP-evoked nociceptor activation. P2RX3 is important for peripheral pain responses, and controls urinary bladder volume reflexes.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for P2X3/P2RX3 Antibody (NB100-1654)(5)

Publications using NB100-1654

• Rhee Y, Choi Y, Hu A et al. Role of Transient Receptor Potential Vanilloid 1 in Sonic Hedgehog-Dependent Taste Bud Differentiation Life 2022-12-27 [PMID: 36676024] (Immunohistochemistry, Mouse)
• Kumari A, Li L, Ermilov AN et al. Unique lingual expression of the Hedgehog pathway antagonist Hedgehog-interacting protein in filiform papillae during homeostasis and ectopic expression in fungiform papillae during Hedgehog signaling inhibition Developmental dynamics : an official publication of the American Association of Anatomists 2022-07-01 [PMID: 35048440] (IHC, Mouse)
• Ermilov AN, Kumari A, Li L et al. Maintenance of Taste Organs Is Strictly Dependent on Epithelial Hedgehog/GLI Signaling. PLoS Genet. 2016-11-01 [PMID: 27893742] (WB, Mouse)
• Kumari A, Ermilov An, Allen Bl et al. Hedgehog pathway blockade with the cancer drug LDE225 disrupts taste organs and taste sensation. J. neurophysiol. 2014-11-12 [PMID: 25392175]
• Tamburro D, Fredolini C, Espina V, Douglas TA, Ranganathan A, Ilag L, Zhou W, Russo P, Espina BH, Muto G, Petricoin EF 3rd, Liotta LA, Luchini A. Multifunctional core-shell nanoparticles: discovery of previously invisible biomarkers. J Am Chem Soc;133(47):19178-88. 2011-11-30 [PMID: 21999289] (IP)

Review for P2X3/P2RX3 Antibody (NB100-1654) (1)

Average Rating: 4

(Based on 1 review)

We have 1 review tested in 1 species: Mouse.

reviewed by: Tamara Haque Flow Mouse 03/13/2018

Summary

• Application: Flow Cytometry
• Sample Tested: Primary mouse cells
• Species: Mouse
• Lot: 402163

Comments

• Comments: Bone marrow derived mouse mast cells were fixed and permeabilized in 4% PFA/saponin and stained with this product at 4 degrees and a secondary antibody, PE anti-rabbit.

Bioinformatics

• Gene Symbol: P2RX3
• Entrez:
  • 5024(Human)
  • 81739(Rat)