p19 INK4d Overexpression Lysate (Native)

Images

 
Western Blot: p19 INK4d Overexpression Lysate (Adult Normal) [NBL1-09057] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for p19 INK4d.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

p19 INK4d Overexpression Lysate (Native) Summary

Immunogen
The lysate was created in HEK293T cells, using plasmid ID RC201155 and based on accession number NM_079421. The protein contains a C-terminal DDK tag.
Specificity
Homo sapiens cyclin-dependent kinase inhibitor 2D (p19, inhibits CDK4) (CDKN2D), transcript variant 2, mRNA.
Gene
CDKN2D

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
17.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for Array

Type
Overexpression
Protein State
Native

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for p19 INK4d Overexpression Lysate (Native)

  • CDK inhibitor p19INK4d
  • cell cycle inhibitor, Nur77 associating protein
  • cyclin-dependent kinase 4 inhibitor D p19
  • cyclin-dependent kinase 4 inhibitor D
  • cyclin-dependent kinase inhibitor 2D (p19, inhibits CDK4)
  • inhibitor of cyclin-dependent kinase 4d
  • INK4D
  • p19
  • p19-INK4D

Background

The protein encoded by this gene is a member of the INK4 family of cyclin-dependent kinase inhibitors. This protein has been shown to form a stable complex with CDK4 or CDK6, and prevent the activation of the CDK kinases, thus function as a cell growth regulator that controls cell cycle G1 progression. The abundance of the transcript of this gene was found to oscillate in a cell-cycle dependent manner with the lowest expression at mid G1 and a maximal expression during S phase. The negative regulation of the cell cycle involved in this protein was shown to participate in repressing neuronal proliferation, as well as spermatogenesis. Two alternatively spliced variants of this gene, which encode an identical protein, have been reported. [provided by RefSeq]. Transcript Variant: This variant (2) contains a different 5' UTR region, and encodes an identical protein, as compared to variant 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Publications for p19 INK4d Lysate (NBL1-09057) (0)

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Bioinformatics

Gene Symbol CDKN2D