p130Cas/BCAR1 Antibody (GT331) - Azide and BSA Free

Western Blot: p130Cas/BCAR1 Antibody (GT331) [NBP3-13565] - Whole cell extract (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with p130Cas/BCAR1 antibody [GT331] (NBP3-13565) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.

Immunohistochemistry-Frozen: p130Cas/BCAR1 Antibody (GT331) [NBP3-13565] - p130Cas/BCAR1 antibody [GT331] detects p130Cas/BCAR1 protein expression by immunohistochemical analysis. Sample: Frozen-sectioned adult mouse cerebellum. Green: p130Cas/BCAR1 protein stained by p130Cas/BCAR1 antibody [GT331] (NBP3-13565) diluted at 1:250. Blue: Fluoroshield with DAPI. Antigen Retrieval: Citrate buffer, pH 6.0, 10 min

Immunohistochemistry-Paraffin: p130Cas/BCAR1 Antibody (GT331) [NBP3-13565] - p130Cas/BCAR1 antibody [GT331] detects p130Cas/BCAR1 protein at cytoplasm in mouse brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse brain. p130Cas/BCAR1 antibody [GT331] (NBP3-13565) diluted at 1:200. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Immunohistochemistry-Paraffin: p130Cas/BCAR1 Antibody (GT331) [NBP3-13565] - p130Cas/BCAR1 antibody [GT331] detects p130Cas/BCAR1 protein at cytoplasm in rat brain by immunohistochemical analysis. Sample: Paraffin-embedded rat brain. p130Cas/BCAR1 antibody [GT331] (NBP3-13565) diluted at 1:200. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

• Reactivity: Hu, Mu, Rt
• Applications: WB, IHC
• Clone: GT331
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

p130Cas/BCAR1 Antibody (GT331) - Azide and BSA Free Summary

• Description: Centrifuge briefly prior to opening.
• Immunogen: Recombinant protein encompassing a sequence within the center region of human p130Cas/BCAR1. The exact sequence is proprietary.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: BCAR1
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin
  • Western Blot

Reactivity Notes

Mouse (88%), Rat (88%), Bovine (88%)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS, 20% Glycerol
• Preservative: No Preservative
• Purity: Protein G purified

Alternate Names for p130Cas/BCAR1 Antibody (GT331) - Azide and BSA Free

• BCAR1
• breast cancer estrogen resistance 1
• Cas scaffolding protein family member 1
• CAS1
• CASFLJ45059
• CASS1
• CASS1CRKASbreast cancer estrogen resistance protein 1
• CRKAS
• Crk-associated substrate p130Cas
• CRK-associated substrate
• FLJ12176
• p130Cas

Background

BCAR1/p130CAS functions as a scaffolding protein for components of various kinase signaling pathways. It is involved in integrin signaling for cell motility as well as netrin signaling for the guidance of commissural axons. Overexpression of BCAR1/p130CAS has been shown to confer antiestrogen resistance in breast cancer cells. Alternate names for BCAR1/p130CAS include breast cancer anti-estrogen resistance protein 1, CRK-associated substrate, CAS scaffolding protein family member 1, CAS, and CRKAS.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: BCAR1