p120-catenin Antibody (6H11)

Product Discontinued

p120-catenin Antibody (6H11) Summary

• Immunogen: NB120-11508 is derived from the 6H11 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an A/J mouse immunized with a recombinant N-terminal fragment of murine delta 1 Catenin.
• Localization: Cytoplasmic
• Specificity: delta 1 Catenin (6H11)
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: CTNND1
• Purity: Tissue culture supernatant

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Immunoprecipitation 1:10-1:500
  • Western Blot 1:10000
• Application Notes: WB: Detects a band of approximately 120 kDa (and a possible doublet band of approximately 200 kDa).

Reactivity Notes

Cross-reacts with Rat, Cow, Chicken, Dog, Human and Mouse. Not yet tested in other species. Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Tissue culture supernatant
• Preservative: 0.09% Sodium Azide
• Purity: Tissue culture supernatant

Alternate Names for p120-catenin Antibody (6H11)

• Cadherin-associated Src substrate
• CAS
• catenin (cadherin-associated protein), delta 1
• catenin delta-1
• CTNND
• KIAA0384P120CAS
• p120 catenin
• p120
• p120(CAS)
• p120(CTN)
• p120cas
• p120ctn

Background

The alpha, beta, delta, and gamma -catenins are proteins that bind to the highly conserved, intracellular cytoplasmic tail of E-cadherin. Together, the catenin/cadherin complexes play an important role mediating cellular adhesion. alpha-catenin, a 102 kDa protein, interacts with E-cadherin associated protein, and also associates with other members of the cadherin family, such as N-cadherin and P-cadherin. The 92 kDa beta-catenin associates with the cytoplasmic portion of E-cadherin, which is necessary for the function of E-cadherin as an adhesion molecule. beta-catenin also complexes with the tumor suppressor protein APC. delta-catenin interacts with Presenilin 1 and is expressed in the brain. The gene encoding delta-catenin maps to human chromosome 5p15.2. A hemizygous loss of the gene encoding delta-catenin leads to the mental retardation associated with Cri-du-Chat syndrome. gamma-catenin, also known as plakoglobin, is an 80-88 kDa protein that binds alpha-catenin and N-cadherin. In addition, the transmembrane phosphatase PTPm associates with catenin/cadherin complexes and may regulate complex signaling.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CTNND1
• Entrez:
  • 1500(Human)
  • 12388(Mouse)
  • 311163(Rat)