OSBPL3 Antibody

Flow Cytometry: OSBPL3 Antibody [NBP2-77443] - Analysis of paraformaldehyde fixed A431 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (1 ug/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.

Immunofluorescence: OSBPL3 Antibody [NBP2-77443] - Analysis of paraformaldehyde fixed A431 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10u g/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing cytoplasmic and some nuclear staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).

• Reactivity: Hu, Mu, Rt, Po, Bv, Ca
• Applications: ELISA, Flow, ICC/IF
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated

OSBPL3 Antibody Summary

• Description: Novus Biologicals Goat OSBPL3 Antibody (NBP2-77443) is a polyclonal antibody validated for use in ELISA, Flow and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Peptide with sequence MSDEKNLGVSQKL-C corresponding to N-Terminus according to NP_056365.1, NP_663160.1, NP_663161.1, NP_663162.1.
• Epitope: MSDEKNLGVSQKL
• Specificity: This antibody is expected to recognise all reported isoforms (NP_056365.1; NP_663160.1; NP_663161.1; NP_663162.1).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: OSBPL3
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 10 ug/mL
  • Immunocytochemistry/ Immunofluorescence
  • Peptide ELISA Detection limit 1:32000
• Application Notes: Western blot application reported in scientific literature (PMID: 22241858).
• Control:

  MeCP2 Antibody (4B6) - Azide and BSA Free

Reactivity Notes

Expected from sequence similarity: Mouse, Rat, Canine, Porcine, Bovine.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for OSBPL3 Antibody

• DKFZp667P1518
• KIAA0704ORP3ORP-3OSBP3
• MGC21526
• OSBP-related protein 3
• oxysterol binding protein-like 3
• oxysterol-binding protein 3
• oxysterol-binding protein-related protein 3

Background

The OSBPL3 gene encodes a member of the oxysterol-binding protein (OSBP) family, a group of intracellular lipid receptors. Most members contain an N-terminal pleckstrin homology domain and a highly conserved C-terminal OSBP-like sterol-binding domain. Several t

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: OSBPL3