NQO-2 Antibody

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Western blot shows lysates of human liver tissue, human kidney tissue, HepG2 human hepatocellular carcinoma cell line, K562 human chronic myelogenous leukemia cell line, and A549 human lung carcinoma cell line. PVDF ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB
Clonality
Polyclonal
Host
Sheep
Conjugate
Unconjugated
Concentration
LYOPH

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Catalog# & Formulation Size Price

NQO-2 Antibody Summary

Immunogen
E. coli-derived recombinant human NQO-2
Ala2-Gln231 (Leu47Phe)
Accession # P16083
Specificity
Detects human NQO-2 in direct ELISAs and Western blots. In direct ELISAs, less than 3% cross-reactivity with recombinant human NQO-1 is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Sheep
Gene
NQO2
Purity
Immunogen affinity purified
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.25 ug/mL

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
Sterile PBS to a final concentration of 0.2 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for NQO-2 Antibody

  • DHQV
  • DIA6
  • dioxin-inducible-2
  • EC 1.10.99.2
  • NAD(P)H dehydrogenase, quinone 2
  • NAD(P)H menadione oxidoreductase 2, dioxin-inducible
  • NMOR2
  • NQO2
  • NQO-2
  • NRH dehydrogenase [quinone] 2
  • NRH:quinone oxidoreductase 2
  • QR2
  • Quinone Reductase 2
  • ribosyldihydronicotinamide dehydrogenase [quinone]
  • ribosyldihydronicotinamide dehydrogenase

Background

NQO-2 (NADPH Quinone acceptor Oxidoreductase 2; also QR2, melatonin receptor 3/MT3 and NRH dehydrogenase) is a 25-26 kDa cytoplasmic flavoprotein member of the NAD(P)H dehydrogenase family of enzymes. It shows restricted expression, being found in retinal pigment epithelium, prostatic fibroblasts, select CNS neurons and RBCs. Like NQO-1, NQO-2 would appear to reduce catechol quinones, but without the use of NADH. In contrast to NQO-1, NQO-2 would appear to activate some quinones, generating cytotoxic products. NQO-2 also reportedly binds and stabilizes key cell protection molecules such as p53, thus acting as a gatekeeper for proteosome-mediated protein turnover. Finally, NQO-2 reportedly binds to melatonin, an act that may promote the subsequent binding of the androgen receptor and an inhibition of cell proliferation. Human NQO-2 is 231 amino acids (aa) in length. It contains a flavodoxin-like domain (aa 4-212) and three utilized Ser phosphorylation sites. NQO-2 functions as a noncovalent homodimer. There are two potential isoform variants. One shows an alternative start site at Met117, while another shows a deletion of aa 102-139. Full length human NQO-2 shares 81% aa sequence identity with mouse NQO-2, and less than 50% aa sequence identity with human NQO-1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol NQO2
Uniprot