NQO-2 Antibody (1E3)

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Western Blot: NQO-2 Antibody (1E3) [NBP1-74033] - Mouse liver lysates (35ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human NQO2 (1:500). Proteins were visualized using a goat ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, ELISA
Clone
1E3
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1 mg/ml

NQO-2 Antibody (1E3) Summary

Immunogen
Recombinant human NQO2 (1-231aa) purified from E. coli
Specificity
Anti-human NQO2 mAb, clone 1E3, is derived from hybridization of mouse FO myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human NQO2 protein.
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
NQO2
Purity
Protein G purified
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Applications/Dilutions

Dilutions
  • Western Blot 1:500
  • ELISA 1:100-1:2000
Application Notes
The antibody has been tested by ELISA and Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended dilution range for Western blot analysis is 1:250 ~ 500. Recommended starting dilution is 1:500

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.4) and 10% Glycerol
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Protein G purified

Alternate Names for NQO-2 Antibody (1E3)

  • DHQV
  • DIA6
  • dioxin-inducible-2
  • EC 1.10.99.2
  • NAD(P)H dehydrogenase, quinone 2
  • NAD(P)H menadione oxidoreductase 2, dioxin-inducible
  • NMOR2
  • NQO2
  • NQO-2
  • NRH dehydrogenase [quinone] 2
  • NRH:quinone oxidoreductase 2
  • QR2
  • Quinone Reductase 2
  • ribosyldihydronicotinamide dehydrogenase [quinone]
  • ribosyldihydronicotinamide dehydrogenase

Background

Quinone oxidoreductase (NQO1 and NQO2) are cytosolic proteins that catalyze metabolic redaction of quinines and derivates.NQO2 is inhibited by flavones such as quercetin. Also benzo(a)pyrene is another known inhibitor of NQO2. Even though overlapping substrates specificities have been observed for NQO1 and NQO2, such as for CB1954 activation, significant differences exist in relative affinities for the various substrates.The detoxification role of NQO2 has not been found, and it has no known endogenous biological substrates. However, NQO1 plays an important role in the detoxification of various endogenous and exogenous quinones, including estrogen quinines. Also NQO2 has a melatonin-binding site, which may explain the anti-oxidant role of melatonin related with circadian rhythm.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol NQO2
Entrez
Uniprot