Monkey Nociceptin ELISA Kit (Colorimetric)

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Standard Curve Reference.
Samples were spiked with high concentrations of Monkey OFQ/N, and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.
Samples were spiked with high concentrations of Monkey Nociceptin, and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Product Details

Summary
Reactivity PmSpecies Glossary
Applications ELISA
Standard Curve Range
4.69 - 300 pg/ml
Sensitivity
2.81 pg/ml

Order Details

Monkey Nociceptin ELISA Kit (Colorimetric) Summary

Description
Assay Length: 2 h 30 min
Standard Curve Range
4.69 - 300 pg/ml
Sensitivity
2.81 pg/ml
Assay Type
Competitive ELISA
Inter-Assay
CV% < 10
Intra-Assay
CV% < 10
Spike Recovery
80 - 120%
Sample Volume
50 uL
Kit Type
ELISA Kit (Colorimetric)
Gene
PNOC

Applications/Dilutions

Dilutions
  • ELISA

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. Biotinylated Detection Ab Diluent
  2. Certificate of Analysis
  3. Concentrated Biotinylated Detection Ab (100x)
  4. Concentrated HRP Conjugate (100x)
  5. Concentrated Wash Buffer (25x)
  6. HRP Conjugate Diluent
  7. Micro ELISA Plate (Dismountable)
  8. Plate Sealer
  9. Product Description
  10. Reference Standard & Sample Diluent
  11. Reference Standard
  12. Stop Solution
  13. Substrate Reagent

Alternate Names for Monkey Nociceptin ELISA Kit (Colorimetric)

  • nociceptin
  • nocistatin
  • OFQ
  • P
  • PPNOC
  • prepronociceptin
  • propronociceptin

Background

Nociceptin (Orphanin FQ, OFQ), a heptadecapeptide peptide, has been designated as an endogenous ligand for the ORL1 receptor. The amino acid sequence of nociceptin hashomology with other opioid peptides, especially the prodynorphin fragment dynorphin A, suggesting a close evolutionary relationship between the precursors. A diffuse distribution throughout the brain is seen with binding studies and in situ hybridization suggesting an extensive role for nociceptin in a multitude of CNS functions. Immunocytochemical localizations in rat spinal cord have demonstrated nociceptin abundance in superficial dorsal horn, lateral spinal nucleus and the region dorsal to the central canal. Nociceptin immunoreactivity was not affected by dorsal rhizotomy, indicating that in spinal cord the peptide is produced by central rather than primary afferent neurons. The localization is supported by its function in processing of nociceptive signals.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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FAQs for Nociceptin ELISA Kit (NBP3-49860). (Showing 1 - 1 of 1 FAQ).

  1. wondering what the difference is between your quantikine and duo set elisas?
    • Usually the duosets do not have the entire kit such as plates and buffers, whereas the other kits are complete.

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Bioinformatics

Gene Symbol PNOC