Rat Nociceptin ELISA Kit (Colorimetric)

ELISA: Rat Nociceptin ELISA Kit (Colorimetric) [NBP3-00482] - Samples were spiked with high concentrations of Rat Nociceptin and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

ELISA: Rat Nociceptin ELISA Kit (Colorimetric) [NBP3-00482] - Standard Curve Reference

• Reactivity: Rt
• Applications: ELISA
• Suitable Sample Type: Serum, plasma, cell supernatant, and other biological fluids
• Standard Curve Range: 15.63 - 1000 pg/mL
• Sensitivity: 9.38 pg/mL

Rat Nociceptin ELISA Kit (Colorimetric) Summary

• Standard Curve Range: 15.63 - 1000 pg/mL
• Sensitivity: 9.38 pg/mL
• Assay Type: Competitive-ELISA
• Inter-Assay: %CV < 5.29
• Intra-Assay: %CV < 6.87
• Spike Recovery: 87 - 105%
• Sample Volume: 100 uL
• Kit Type: ELISA Kit (Colorimetric)
• Gene: PNOC

Applications/Dilutions

• Dilutions:
  • ELISA

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Alternate Names for Rat Nociceptin ELISA Kit (Colorimetric)

• nociceptin
• nocistatin
• OFQ
• P
• PPNOC
• prepronociceptin
• propronociceptin

Background

Nociceptin (Orphanin FQ, OFQ), a heptadecapeptide peptide, has been designated as an endogenous ligand for the ORL1 receptor. The amino acid sequence of nociceptin hashomology with other opioid peptides, especially the prodynorphin fragment dynorphin A, suggesting a close evolutionary relationship between the precursors. A diffuse distribution throughout the brain is seen with binding studies and in situ hybridization suggesting an extensive role for nociceptin in a multitude of CNS functions. Immunocytochemical localizations in rat spinal cord have demonstrated nociceptin abundance in superficial dorsal horn, lateral spinal nucleus and the region dorsal to the central canal. Nociceptin immunoreactivity was not affected by dorsal rhizotomy, indicating that in spinal cord the peptide is produced by central rather than primary afferent neurons. The localization is supported by its function in processing of nociceptive signals.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

FAQs for Nociceptin ELISA Kit (NBP3-00482). (Showing 1 - 1 of 1 FAQ).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: PNOC