Human Nociceptin ELISA Kit (Colorimetric)

ELISA: Human Nociceptin ELISA Kit (Colorimetric) [NBP2-81196] - Samples were spiked with high concentrations of Human Nociceptin and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

ELISA: Human Nociceptin ELISA Kit (Colorimetric) [NBP2-81196] - Standard Curve Reference

• Reactivity: Hu
• Applications: ELISA
• Suitable Sample Type: Serum, plasma, cell supernatant, and other biological fluids
• Standard Curve Range: 4.69 - 300 pg/mL
• Sensitivity: 2.81 pg/mL

Human Nociceptin ELISA Kit (Colorimetric) Summary

• Specificity: This kit recognizes Human Nociceptin in samples. No significant cross-reactivity or interference between Human Nociceptin and analogues was observed.
• Standard Curve Range: 4.69 - 300 pg/mL
• Sensitivity: 2.81 pg/mL
• Assay Type: Sandwich-ELISA
• Inter-Assay: CV% < 4.67%
• Intra-Assay: CV% < 5.23%
• Spike Recovery: 88-107%
• Sample Volume: 100 ul
• Kit Type: ELISA Kit (Colorimetric)
• Gene: PNOC

Applications/Dilutions

• Dilutions:
  • ELISA

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Alternate Names for Human Nociceptin ELISA Kit (Colorimetric)

• nociceptin
• nocistatin
• OFQ
• P
• PPNOC
• prepronociceptin
• propronociceptin

Background

Nociceptin (Orphanin FQ, OFQ), a heptadecapeptide peptide, has been designated as an endogenous ligand for the ORL1 receptor. The amino acid sequence of nociceptin hashomology with other opioid peptides, especially the prodynorphin fragment dynorphin A, suggesting a close evolutionary relationship between the precursors. A diffuse distribution throughout the brain is seen with binding studies and in situ hybridization suggesting an extensive role for nociceptin in a multitude of CNS functions. Immunocytochemical localizations in rat spinal cord have demonstrated nociceptin abundance in superficial dorsal horn, lateral spinal nucleus and the region dorsal to the central canal. Nociceptin immunoreactivity was not affected by dorsal rhizotomy, indicating that in spinal cord the peptide is produced by central rather than primary afferent neurons. The localization is supported by its function in processing of nociceptive signals.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

Bioinformatics

• Gene Symbol: PNOC