Nociceptin Antibody

Immunohistochemistry: Nociceptin Antibody [NB100-1659] - Immunohistochemistry: This orphanin FQ robustly stains nerve fibers in brain and spinal cord. Animals were treated with colchicines to visualize orphanin FQ-expressing cell bodies. Staining was blocked by preabsorption with the cognate peptide.

Product Discontinued

Nociceptin Antibody Summary

• Immunogen: Synthetic peptide: FGGFTGARKSARKLANQ, corresponding to amino acids 122-138 of Rat Orphanin FQ.
• Localization: Secreted
• Specificity: Nociceptin
• Isotype: IgG
• Clonality: Polyclonal
• Host: Guinea Pig
• Gene: PNOC
• Purity: Unpurified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: Whole antisera
• Preservative: 0.05% Sodium Azide
• Purity: Unpurified

Alternate Names for Nociceptin Antibody

• nociceptin
• nocistatin
• OFQ
• P
• PPNOC
• prepronociceptin
• propronociceptin

Background

Nociceptin (Orphanin FQ, OFQ), a heptadecapeptide peptide, has been designated as an endogenous ligand for the ORL1 receptor. The amino acid sequence of nociceptin hashomology with other opioid peptides, especially the prodynorphin fragment dynorphin A, suggesting a close evolutionary relationship between the precursors. A diffuse distribution throughout the brain is seen with binding studies and in situ hybridization suggesting an extensive role for nociceptin in a multitude of CNS functions. Immunocytochemical localizations in rat spinal cord have demonstrated nociceptin abundance in superficial dorsal horn, lateral spinal nucleus and the region dorsal to the central canal. Nociceptin immunoreactivity was not affected by dorsal rhizotomy, indicating that in spinal cord the peptide is produced by central rather than primary afferent neurons. The localization is supported by its function in processing of nociceptive signals.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PNOC
• Entrez:
  • 25516(Rat)