Nociceptin Antibody

Immunohistochemistry: Nociceptin Antibody [NB100-1619] - This orphanin FQ robustly stains nerve fibers in brain and spinal cord. Animals were treated with colchicines to visualize orphanin FQ-expressing cell bodies. Staining was blocked by preabsorption with the cognate peptide.

Product Discontinued

Nociceptin Antibody Summary

• Immunogen: FGGFTGARKSARKLANQ- Peptide sequence corresponds to: amino acids 135-151 of rat, 154-170 of mouse and 130-146 of human pro-nociceptin.
• Localization: Secreted
• Specificity: Nociceptin
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: PNOC
• Purity: Unpurified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Western Blot 1:100-1:2000

Reactivity Notes

Immunogen displays the 100% of sequence identity for a wide range of non-tested species.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: Whole antisera
• Preservative: 0.05% Sodium Azide
• Purity: Unpurified

Alternate Names for Nociceptin Antibody

• nociceptin
• nocistatin
• OFQ
• P
• PPNOC
• prepronociceptin
• propronociceptin

Background

Orphanin FQ (nociceptin, OFQ), a heptadecapeptide peptide, has been designated as an endogenous ligand for the ORL1 receptor. The amino acid sequence of OFQ has homology with other opioid peptides, especially the prodynorphin fragment dynorphin A, suggesting a close evolutionary relationship between the precursors. A diffuse distribution throughout the brain is seen with binding studies and in situ hybridization suggesting an extensive role for OFQ in a multitude of CNS functions. Immunocytochemical localizations in rat spinal cord have demonstrated OFQ abundance in superficial dorsal horn, lateral spinal nucleus and the region dorsal to the central canal. OFQ immunoreactivity was not affected by dorsal rhizotomy, indicating that in spinal cord the peptide is produced by central rather than primary afferent neurons. The localization is supported by its function in processing of nociceptive signals.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Nociceptin Antibody (NB100-1619)(2)

Publications using NB100-1619

• Kallupi M, Carrette LLG, Kononoff J et al. Nociceptin attenuates the escalation of oxycodone self-administration by normalizing CeA-GABA transmission in highly addicted rats Proc. Natl. Acad. Sci. U.S.A. 2020-01-28 [PMID: 31932450] (WB, Rat)
• Ulugol A, Topuz RD, Gunduz O et al. Changes in nociceptin/orphanin FQ levels in rat brain regions after acute and chronic cannabinoid treatment in conjunction with the development of antinociceptive tolerance Fundam Clin Pharmacol 2016-07-01 [PMID: 27371029] (WB, IF/IHC)

FAQs for Nociceptin Antibody (NB100-1619). (Showing 1 - 1 of 1 FAQs).

1. Which permeabilisation buffer would you recommend for intracellular staining using NB100-1619 for flow cytometry?
   • You can use whatever you lab standard is for permeabilization. Our lab uses 90% methanol. However, I would like to note that flow cytometry has been removed from our list of guaranteed applications. This was erroneously listed on the datasheet and has never been directly tested in flow cytometry although it is expected to work in flow.

Bioinformatics

• Gene Symbol: PNOC
• Entrez:
  • 25516(Rat)