NKD2 Antibody

Product Discontinued

NKD2 Antibody Summary

• Immunogen: Peptide with sequence C-HKRYRQKGREGHS corresponding to internal region according to NP_149111.1.
• Epitope: C-HKRYRQKGREGHS
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: NKD2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Peptide ELISA Detection limit 1:32000
• Application Notes: WB: Preliminary experiments gave bands at approx. 80 kDa, 38 kDa band and 26 kDa band in mouse brain lysates after 0.1 ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe given the calculated size of 51.5 kDa band according to mouse NP_082462.2 and of 50.1 kDa band according to human NP_149111.1. All the detected bands were successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the additional bands).

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Notes

Please note, this antibody is considered Innovators Grade. Innovators Grade antibodies are generally unvalidated and require additional characterization for most new species/applications. Novus has made these antibodies available through our Innovators Reward program. Complete an online review with image, detailing your positive or negative results. In return, you receive a discount voucher for 100% of the purchase price of the reviewed product. Please contact us at innovators@novusbio.com for more details.

Alternate Names for NKD2 Antibody

• Dvl-binding protein NKD2
• hNkd2
• naked cuticle homolog 2 (Drosophila)
• naked cuticle-2
• Naked2
• naked-2
• protein naked cuticle homolog 2

Background

In the mouse, Nkd is a Dishevelled (see DVL1; MIM 601365)-binding protein that functions as a negative regulator of the Wnt (see WNT1; MIM 164820)-beta-catenin (see MIM 116806)-Tcf (see MIM 602272) signaling pathway.[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for NKD2 Antibody (NBP1-06058)(1)

Publication using NBP1-06058

• Zhang S, Cagatay T, Amanai M et al. Viable mice with compound mutations in the Wnt/Dvl pathway antagonists nkd1 and nkd2. Mol Cell Biol 2007-06-01 [PMID: 17438140]

Bioinformatics

• Gene Symbol: NKD2
• Entrez:
  • 85409(Human)
  • 72293(Mouse)
  • 308068(Rat)
• Uniprot:
  • NP_149111.1(Human)