NDUFS8 Antibody

Western Blot: NDUFS8 Antibody [NBP2-26107] - Staining of Mouse Heart lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

• Reactivity: Hu, Mu, Rt, Bv, Ca
• Applications: WB, ELISA
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

NDUFS8 Antibody Summary

• Description: Novus Biologicals Goat NDUFS8 Antibody (NBP2-26107) is a polyclonal antibody validated for use in WB and ELISA. Anti-NDUFS8 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Peptide with sequence C-AEPRADGSRR corresponding to internal region according to NP_002487.1.
• Predicted Species: Rat (100%), Canine (100%), Bovine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: NDUFS8
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Peptide ELISA Detection limit 1:2000
  • Western Blot 0.1 - 0.3 ug/ml
• Application Notes: WB: Approx. 25 kDa band observed in human and mouse heart lysates (calculated MW of 23.7 kDa band according to NP_002487.1). An additional fainter band of 90 kDa band was consistently observed, however this band was not blocked by the immunizing peptide and it is therefore a non-specific signal. We call for caution when used for other assays than WB.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for NDUFS8 Antibody

• CI-23k
• CI23KD
• CI-23kD
• complex I 23kDa subunit
• Complex I-23kD
• EC 1.6.5.3
• EC 1.6.99.3
• EC 1.6.99.5
• NADH dehydrogenase (ubiquinone) Fe-S protein 8 (23kD) (NADH-coenzyme Qreductase)
• NADH dehydrogenase (ubiquinone) Fe-S protein 8, 23kDa (NADH-coenzyme Qreductase)
• NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial
• NADH-ubiquinone oxidoreductase 23 kDa subunit
• TYKY subunit
• TYKY

Background

NDUFS8 (NADH dehydrogenase (ubiquinone) Fe-S protein 8, 23kDa (NADH-coenzyme Q reductase)) is a subunit of NADH. Mutations in NDUFS8 have been linked to Leigh syndrome.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for NDUFS8 Antibody (NBP2-26107)(1)

Publication using NBP2-26107

• Saito A, Kawamoto M, Kamatani N. Association study between single-nucleotide polymorphisms in 199 drug-related genes and commonly measured quantitative traits of 752 healthy Japanese subjects. J Hum Genet 2009-06-01 [PMID: 19343046]

Bioinformatics

• Gene Symbol: NDUFS8
• Entrez:
  • 4728(Human)
  • 225887(Mouse)
  • 293652(Rat)