NDUFS3 Knockout 293T Cell Lysate

Images

 

Product Details

Summary
Product Discontinued
View other related NDUFS3 Lysates

Order Details


    • Catalog Number
      NBP2-65754
    • Availability
      Product Discontinued

    Can't find what you are looking for? Use our Antibody Concierge Service & we will help you locate your antibody!

    Or feel free to contact us for alternative products.

NDUFS3 Knockout 293T Cell Lysate Summary

Preparation
Method
Knockout achieved by using CRISPR/Cas9,19 bp deletion in exon 1
Gene
NDUFS3

Applications/Dilutions

Dilutions
  • Western Blot
Application Notes
You will receive 1 vial (100ug) of knockout cell lysate and 1 vial (100ug) of Parental cell lysate. Lysate can be diluted with 1X SDS sample buffer and will be stable at -20 degrees C for 12 months. Minimize freeze-thaw cycles.

Packaging, Storage & Formulations

Storage
Store at -20C short term. Aliquot and store at -80C long term. Avoid freeze-thaw cycles.
Buffer
0.1 mg cell homogenate lyophilized in RIPA buffer made with double-knockout cell lines.
Concentration
LYOPH
Reconstitution Instructions
To use as WB negative control, spin down briefly and resuspend in 100 uL 1xSDS sample buffer (2% SDS, 60 mM Tris-HCl pH 6.8, 10% Glycerol, 0.02% Bromophenol blue, 60 mM beta-mercaptoethanol). Boil the lysate for 3 - 5 minutes before loading it onto gel.

Lysate Details for Array

Type
Knockout 293T Cell
Life Stage
Embryonic
Tissue
Kidney

Notes

Powered by EDIGENE.
Validation of antibody specificity is critical and verification of antibody performance against knockout samples is one way to guarantee that an antibody recognizes a specific target. Novus' KO cell lysate can be used as a negative control for western blots and to confirm the specificity of antibodies.

Alternate Names for NDUFS3 Knockout 293T Cell Lysate

  • CI-30
  • CI-30kD
  • complex I 30kDa subunit
  • Complex I-30kD
  • EC 1.6.5.3
  • EC 1.6.99.3
  • EC 1.6.99.5
  • NADH dehydrogenase (ubiquinone) Fe-S protein 3 (30kD) (NADH-coenzyme Qreductase)
  • NADH dehydrogenase (ubiquinone) Fe-S protein 3, 30kDa (NADH-coenzyme Qreductase)
  • NADH dehydrogenase [ubiquinone] iron-sulfur protein 3, mitochondrial
  • NADH dehydrogenase-ubiquinone 30 kDa subunit
  • NADH-ubiquinone oxidoreductase 30 kDa subunit

Background

This gene encodes one of the iron-sulfur protein (IP) components of mitochondrial NADH:ubiquinone oxidoreductase (complex I). Mutations in this gene are associated with Leigh syndrome resulting from mitochondrial complex I deficiency.[provided by RefSeq, Apr 2009]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Customers Who Viewed This Item Also Viewed...

NBP1-90050
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC,  IHC-P, WB
NBP1-92172
Species: Hu
Applications: ICC/IF, IHC,  IHC-P, WB
NBP1-33074
Species: Hu, Mu, Ze
Applications: IHC,  IHC-P, KO, WB
NBP1-84475
Species: Hu, Mu
Applications: IHC,  IHC-P, WB
NBP1-31142
Species: Hu, Mu, Rt
Applications: IHC,  IHC-P, WB
NBP2-46127
Species: Hu, Mu, Rt
Applications: IHC,  IHC-P, KO, WB
AF2905
Species: Hu
Applications: ELISA, ICC, WB
NBP2-45645
Species: Hu
Applications: ICC/IF, IHC,  IHC-P, WB
AF3470
Species: Hu
Applications: ICC, WB
NB110-60531
Species: Hu, Mu, Rt(-)
Applications: CyTOF-ready, ELISA, Flow-IC, Flow, ICC/IF, IHC,  IHC-P, IP, WB
NBP2-93743
Species: Hu, Mu, Rt
Applications: ICC/IF, WB
NBP1-31465
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC,  IHC-P, WB
NBP1-85620
Species: Hu
Applications: ICC/IF, IHC,  IHC-P
NBP1-91228
Species: Hu, Rt
Applications: IHC,  IHC-P, WB
NBP1-33021
Species: Hu, Mu
Applications: ICC/IF, IHC,  IHC-P, IP, KO, WB
NB100-56618
Species: Hu, Mu
Applications: Flow-CS, Flow, ICC/IF, IHC,  IHC-P, WB
NBP1-49846
Species: Hu
Applications: IHC,  IHC-P, PEP-ELISA, WB
NBP2-65754
Species: Hu
Applications: WB

Publications for NDUFS3 Lysate (NBP2-65754) (0)

There are no publications for NDUFS3 Lysate (NBP2-65754).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for NDUFS3 Lysate (NBP2-65754) (0)

There are no reviews for NDUFS3 Lysate (NBP2-65754). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for NDUFS3 Lysate (NBP2-65754) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional NDUFS3 Products

Research Areas for NDUFS3 Lysate (NBP2-65754)

Find related products by research area.

Blogs on NDUFS3

There are no specific blogs for NDUFS3, but you can read our latest blog posts.

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our NDUFS3 Knockout 293T Cell Lysate and receive a gift card or discount.

Bioinformatics

Gene Symbol NDUFS3