NDUFS3 Antibody

Western Blot: NDUFS3 Antibody [NBP2-76953] - Western blot analysis of NDUFS3 on different cell lysates using anti-NDUFS3 antibody at 1/500 dilution.Positive control:Lane1: LOVOLane2: MCF-7

Immunohistochemistry: NDUFS3 Antibody [NBP2-76953] - Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-NDUFS3 antibody. Counter stained with hematoxylin.

Flow Cytometry: NDUFS3 Antibody [NBP2-76953] - Flow cytometric analysis of MCF-7 cells with NDUFS3 antibody at 1/100 dilution (fuchsia) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.

Immunohistochemistry: NDUFS3 Antibody [NBP2-76953] - Immunohistochemical analysis of paraffin-embedded rat epididymis tissue using anti-NDUFS3 antibody. Counter stained with hematoxylin.

Immunohistochemistry: NDUFS3 Antibody [NBP2-76953] - Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti-NDUFS3 antibody. Counter stained with hematoxylin.

Product Discontinued

NDUFS3 Antibody Summary

• Immunogen: Recombinant protein within Human NDUFS3 aa 7-239 / 264. (SwissProt: O75489 Human; SwissProt: Q9DCT2 Mouse)
• Localization: Mitochondrion inner membrane.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: NDUFS3
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:50-1:100
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Western Blot 1:500-1:1000
• Theoretical MW: 30 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH7.4), 0.2% BSA, 50% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for NDUFS3 Antibody

• CI-30
• CI-30kD
• complex I 30kDa subunit
• Complex I-30kD
• EC 1.6.5.3
• EC 1.6.99.3
• EC 1.6.99.5
• NADH dehydrogenase (ubiquinone) Fe-S protein 3 (30kD) (NADH-coenzyme Qreductase)
• NADH dehydrogenase (ubiquinone) Fe-S protein 3, 30kDa (NADH-coenzyme Qreductase)
• NADH dehydrogenase [ubiquinone] iron-sulfur protein 3, mitochondrial
• NADH dehydrogenase-ubiquinone 30 kDa subunit
• NADH-ubiquinone oxidoreductase 30 kDa subunit

Background

The multisubunit NADH:ubiquinone oxidoreductase (complex I) is the first enzyme complex in the electron transport chain of mitochondria. The iron-sulfur protein (IP) fraction of complex I is made up of 7 subunits. See NDUFS1 (MIM 157655).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: NDUFS3