Myosin VIIa Antibody

Western Blot: Myosin VIIa Antibody [NB120-3481] - Analysis of mouse testes tissue extract.

Product Discontinued

Myosin VIIa Antibody Summary

• Immunogen: Synthetic Peptide: S(16) G Q E F D V P I G A V V K L C(31)
• Specificity: Detects Myosin VIIa from mouse tissues as well as recombinant protein.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: MYO7A
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Western Blot 5 ug/ml
• Application Notes: WB: Detects an approx. 220 kDa protein representing myosin VIIa from mouse testes preparations. Detects recombinant mouse myosin VIIa overexpressed in Sf9 insect cell lysate. Use in Immunohistochemistry reported in scientific literature (PMID:31895697).
• Theoretical MW: 254 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Guinea Pig

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS with 1 mg/ml BSA
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Myosin VIIa Antibody

• deafness, autosomal dominant 11
• deafness, autosomal recessive 2
• DFNA11
• DFNB2
• myosin VIIA
• myosin-VIIa
• MYOVIIA
• MYU7A
• USH1Bsevere))

Background

Myosin VIIa is a member of the myosin superfamily of actin-based motor proteins. Defects in the myosin VIIa gene are responsible for hearing impairment in shaker-1 (sh1) mice and causes Usher syndrome IB in humans. Usher syndrome associates congenital deafness, vestibular dysfunction, and retinitis pigmentosa and is the most common form of combined deafness and blindness. Structural features of myosin VIIa protein include an ATP binding N-terminal motor domain, a central region which possess five light-chain binding (IQ) motifs, and a C-terminal domain with three myosin tail homology (MyTH4) and talin-like homology regions.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Myosin VIIa Antibody (NB120-3481)(4)

Publications using NB120-3481

• Lin Y, Lin Y, Chen H et al. Ultrasound Microbubbles Enhance the Efficacy of Insulin-Like Growth Factor-1 Therapy for the Treatment of Noise-Induced Hearing Loss Molecules 2021-06-13 [PMID: 34199327] (IF/IHC, Guinea Pig)
• Malfeld K, Armbrecht N, Volk HA et al. In Situ 3D-Imaging of the Inner Ear Synapses with a Cochlear Implant Life (Basel, Switzerland) 2021-04-01 [PMID: 33915846] (IF/IHC, Guinea Pig)
• Liao AH, Wang CH, Weng PY et al. Ultrasound-induced microbubble cavitation via a transcanal or transcranial approach facilitates inner ear drug delivery JCI Insight 2020-01-02 [PMID: 31895697] (IF/IHC, Guinea Pig)
• Tan BT, Lee MM, Ruan R. Bone-marrow-derived cells that home to acoustic deafened cochlea preserved their hematopoietic identity. J Comp Neurol;509(2):167-79. 2008-07-10 [PMID: 18461607]

Bioinformatics

• Gene Symbol: MYO7A
• Entrez:
  • 4647(Human)
  • 17921(Mouse)
  • 397373(Porcine)
• Uniprot:
  • P97479(Mouse)