MYO19 Antibody (JE64-30)

Western Blot: MYO19 Antibody (JE64-30) [NBP3-32628] - Western blot analysis of MYO19 on different lysates with Rabbit anti-MYO19 antibody at 1/500 dilution. Lane 1: HeLa cell lysate Lane 2: A549 cell lysate Lane 3: K562 cell lysate Lysates/proteins at 10 ug/Lane. Predicted band size: 109 kDa Observed band size: 109 kDa Exposure time: 1 minute; 10 % SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody MYO19 at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.

Immunocytochemistry/ Immunofluorescence: MYO19 Antibody (JE64-30) [NBP3-32628] - Immunocytochemistry analysis of NCI-H441 cells labeling MYO19 with Rabbit anti-MYO19 antibody (NBP3-32628) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-MYO19 antibody (NBP3-32628) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/200 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 647) were used as the secondary antibody at 1/1,000 dilution.

• Reactivity: Hu
• Applications: WB, ICC/IF
• Clone: JE64-30
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 1 mg/ml

MYO19 Antibody (JE64-30) Summary

• Description: Novus Biologicals Rabbit MYO19 Antibody (JE64-30) (NBP3-32628) is a recombinant monoclonal antibody validated for use in WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: Recombinant protein within human MYO19 aa 431-680/970. (Uniprot: Q96H55)
• Localization: Cytoskeleton, Mitochondrion outer membrane.
• Source: HEK293
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: MYO19
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:50
  • Western Blot 1:500
• Theoretical MW: 109 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 1*TBS (pH7.4), 0.05% BSA and 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for MYO19 Antibody (JE64-30)

• FLJ22865
• MYOHD1
• myosin head domain containing 1
• Myosin head domain-containing protein 1
• myosin XIX
• myosin-XIX

Background

Myosins are actin-based motor molecules with ATPase activity. Unconventional myosins serve in intracellularmovements. Their highly divergent tails are presumed to bind to membranous compartments, which would be moved relativeto actin filaments

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: MYO19