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Recombinant Human Muscarinic Acetylcholine Receptor M4/CHRM4 Protein

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications AP

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Recombinant Human Muscarinic Acetylcholine Receptor M4/CHRM4 Protein Summary

Description
An untagged recombinant protein corresponding to the amino acid sequence of (NP_000732.2) for Human Muscarinic Acetylcholine Receptor M4/CHRM4

Source: Wheat Germ (in vitro) with proprietary liposome technology

Amino Acid Sequence: MANFTPVNGSSGNQSVRLVTSSSHNRYETVEMVFIATVTGSLSLVTVVGNILVMLSIKVNRQLQTVNNYFLFSLACADLIIGAFSMNLYTVYIIKGYWPLGAVVCDLWLALDYVVSNASVMNLLIISFDRYFCVTKPLTYPARRTTKMAGLMIAAAWVLSFVLWAPAILFWQFVVGKRTVPDNQCFIQFLSNPAVTFGTAIAAFYLPVVIMTVLYIHISLASRSRVHKHRPEGPKEKKAKTLAFLKSPLMKQSVKKPPPGEAAREELRNGKLEEAPPPALPPPPRPVADKDTSNESSSGSATQNTKERPATELSTTEATTPAMPAPPLQPRALNPASRWSKIQIVTKQTGNECVTAIEIVPATPAGMRPAANVARKFASIARNQVRKKRQMAARERKVTRTIFAILLAFILTWTPYNVMVLVNTFCQSCIPDTVWSIGYWLCYVNSTINPACYALCNATFKKTFRHLLLCQYRNIGTAR

Preparation
Method
in vitro wheat germ expression system with proprietary liposome technology
Details of Functionality
This protein was produced in an in vitro wheat germ expression system that should preserve correct conformational folding that is necessary for biological function. While it is possible that this protein could display some level of activity, the functionality of this protein has not been explicitly measured or validated.
Source
Wheat germ
Protein/Peptide Type
Full Length Recombinant Protein
Gene
CHRM4
Purity
>80% by SDS-PAGE and Coomassie blue staining

Applications/Dilutions

Dilutions
  • Immunoaffinity Purification
Theoretical MW
53 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
25 mM Tris-HCl pH8.0 in 2% glycerol.
Preservative
Glycerol
Purity
>80% by SDS-PAGE and Coomassie blue staining

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Recombinant Human Muscarinic Acetylcholine Receptor M4/CHRM4 Protein

  • cholinergic receptor, muscarinic 4
  • CHRM4
  • HM4
  • M4
  • M4R
  • mAChR M4
  • muscarinic acetylcholine receptor M4

Background

Muscarinic acetylcholine receptor M4 is an Acetylcholine Receptor. Unlike muscarinic receptor M2, the M4 receptor is not involved in smooth muscle contraction. The clinical relevance of this receptor is unknown. However, mouse studies have linked it to adenylyl cyclase inhibition. Muscarinic acetylcholine receptor M4 has been reported to be expressed in many regions of the rat brain, particularly the striatum and hippocampus. ESTs have been isolated from human breast and eye libraries.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

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Bioinformatics

Gene Symbol CHRM4