MRPL43 Antibody - Azide and BSA Free

Western Blot: MRPL43 Antibody [H00084545-B01P] - Analysis of MRPL43 expression in transfected 293T cell line by MRPL43 polyclonal antibody. Lane 1: MRPL43 transfected lysate(17.49 KDa). Lane 2: Non-transfected lysate.

• Reactivity: Hu
• Applications: WB
• Clonality: Polyclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

MRPL43 Antibody - Azide and BSA Free Summary

• Description: Novus Biologicals Mouse MRPL43 Antibody - Azide and BSA Free (H00084545-B01P) is a polyclonal antibody validated for use in WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: MRPL43 (NP_115488.2, 1 a.a. - 159 a.a.) full-length human protein. MTARGTPSRFLASVLHNGLGRYVQQLQRLSFSVSRDGASSRGAREFVEREVIDFARRNPGVVIYVNSRPCCVPRVVAEYLNGAVREESIHCKSVEEISTLVQKLADQSGLDVIRIRKPFHTDNPSIQGQWHPFTNKPTTFRGLRPREVQDPAPAQVQAQ
• Specificity: MRPL43 - mitochondrial ribosomal protein L43,
• Isotype: IgG
• Clonality: Polyclonal
• Host: Mouse
• Gene: MRPL43
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:500
• Application Notes: Antibody reactive against transfected lysate for western blot and recombinant protein with GST tag for ELISA and Western Blot. GST tag alone is used as a negative control.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.4)
• Preservative: No Preservative
• Purity: Protein A purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for MRPL43 Antibody - Azide and BSA Free

• 39S ribosomal protein L43, mitochondrial
• bMRP36a
• L43mt
• MGC17989
• MGC48892
• Mitochondrial ribosomal protein bMRP36a
• mitochondrial ribosomal protein L43
• MRP-L43

Background

Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein. This gene and the gene for a semaphorin class 4 protein (SEMA4G) overlap at map location 10q24.31 and are transcribed in opposite directions. Sequence analysis identified multiple transcript variants encoding at least four different protein isoforms. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: MRPL43
• Entrez:
  • 84545(Human)
• Uniprot:
  • NP_115488.2(Human)