MFAP3 Antibody

Western Blot: MFAP3 Antibody [NBP1-86067] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Negative control (vector only transfected HEK293T lysate). Lane 3: Over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).

Immunohistochemistry-Paraffin: MFAP3 Antibody [NBP1-86067] - Staining of human cerebral cortex shows strong cytoplasmic positivity in neuronal cells.

Western Blot: MFAP3 Antibody [NBP1-86067] - Analysis in human cell line RT-4 and human cell line U-251 MG.

• Reactivity: Hu
• Applications: WB, IHC, IHC-P
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

MFAP3 Antibody Summary

• Immunogen: This antibody was developed against Recombinant Protein corresponding to amino acids: EFARYIEELARSVPLPPLILNCRAFVEEMFEAVRVDDPDDLGERIKERPALNAQGGIYVINPEMGRSNSPGGDSDDGSLNEQGQEIAVQVSVHLQSETKSIDTESQGSSHFSPPDDIGSAESNCNYKDGA
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: MFAP3
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:20 - 1:50
  • Immunohistochemistry-Paraffin 1:20 - 1:50
  • Western Blot 0.04-0.4 ug/ml
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended.
• Control Peptide:

  MFAP3 Recombinant Protein Antigen (NBP1-86067PEP)

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (85%), Rat (85%)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for MFAP3 Antibody

• DKFZp586F0219
• MFAP3
• microfibril-associated glycoprotein 3
• microfibrillar-associated protein 3

Background

MFAP3, also known as Microfibril-associated glycoprotein 3, has 2 isoforms, a 362 amino acid long isoform that is 40 kDa and a short 216 amino acid isoform that is 24 kDa, acts as a component of the elastin-associated microfibrils. Little is currently known about this protein disease involvement and interacting proteins.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: MFAP3