MEX3D Antibody - BSA Free

Western Blot: MEX3D Antibody [NBP1-77332] - MDA-MB-361 cell lysate with MEX3D antibody at (A) 1 ug/ml and (B) 2 ug/mL.

Immunohistochemistry-Paraffin: MEX3D Antibody [NBP1-77332] - Human small intestine tissue with MEX3D antibody at 2.5 ug/ml.

Immunocytochemistry/ Immunofluorescence: MEX3D Antibody - BSA Free [NBP1-77332] - Immunofluorescence of rkhd1 in human small intestine tissue with rkhd1 antibody at 20 ug/mL.

• Reactivity: Hu
• Applications: WB, ELISA, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

MEX3D Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit MEX3D Antibody - BSA Free (NBP1-77332) is a polyclonal antibody validated for use in IHC, WB, ELISA and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Antibody was raised against a 15 amino acid synthetic peptide from near the center of human Rkhd1. The immunogen is located within amino acids 360 - 410 of Rkhd1. Amino Acid Squence: WAKTPNQGRRPPTAT
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: MEX3D
• Purity: Peptide affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Immunocytochemistry/ Immunofluorescence 20 ug/ml
  • Immunohistochemistry 2.5 ug/ml
  • Immunohistochemistry-Paraffin 2.5 ug/ml
  • Western Blot 1-2 ug/ml
• Control Peptide:

  MEX3D Peptide (NBP1-77332PEP)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Peptide affinity purified

Alternate Names for MEX3D Antibody - BSA Free

• KIAA2031Tino
• mex-3 homolog D (C. elegans)
• OK/SW-cl.4
• ring finger (C3HC4 type) and KH domain containing 1
• ring finger and KH domain containing 1
• RING finger and KH domain-containing protein 1
• RING finger protein 193
• RKHD1MEX3
• RNA-binding protein MEX3D
• RNF193MEX-3D
• TINObcl-2 ARE RNA binding protein

Background

Rkhd1, also known as TINO or MEX3D is a member of a novel family of four homologous human MEX3 proteins each containing two heterogeneous nuclear ribonucleoprotein K homology (KH) domains and one carboxy-terminal RING finger module. MEX3 proteins, including Rkhd1, are phosphoproteins that bind RNA through their KH domains and shuttle between the nucleus and the cytoplasm via the CRM1 export pathway. These proteins are a novel family of evolutionarily conserved RNA-binding proteins, differentially recruited to P bodies and potentially involved in post-transcriptional regulatory mechanisms. Rkhd1 binds to the AU-rich element in the 3'-untranslated region of Bcl-2 mRNA and is thought to be a negative regulator of Bcl-2 expression. Rkhd3 and Rkhd4, but not Rkhd1, co-localize with both the hDcp1a decapping factor and Argonaute (Ago) proteins in processing bodies (P bodies), recently characterized as centers of mRNA turnover.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: MEX3D
• Uniprot:
  • Q86XN8(Human)