MED15 Knockout HeLa Cell Lysate

Product Discontinued

MED15 Knockout HeLa Cell Lysate Summary

• Preparation Method: Knockout achieved by using CRISPR/Cas9,1 bp deletion in exon 5 and Insertion of the selection cassette in exon 5
• Gene: MED15

Applications/Dilutions

• Dilutions:
  • Western Blot
• Application Notes: You will receive 1 vial (100ug) of knockout cell lysate and 1 vial (100ug) of Parental cell lysate. Lysate can be diluted with 1X SDS sample buffer and will be stable at -20 degrees C for 12 months. Minimize freeze-thaw cycles.

Packaging, Storage & Formulations

• Storage: Store at -20C short term. Aliquot and store at -80C long term. Avoid freeze-thaw cycles.
• Buffer: 0.1 mg cell homogenate lyophilized in RIPA buffer made with double-knockout cell lines.
• Concentration: LYOPH
• Reconstitution Instructions: To use as WB negative control, spin down briefly and resuspend in 100 uL 1xSDS sample buffer (2% SDS, 60 mM Tris-HCl pH 6.8, 10% Glycerol, 0.02% Bromophenol blue, 60 mM beta-mercaptoethanol). Boil the lysate for 3 - 5 minutes before loading it onto gel.

Lysate Details for Array

• Type: Knockout HeLa Cell

Notes

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Validation of antibody specificity is critical and verification of antibody performance against knockout samples is one way to guarantee that an antibody recognizes a specific target. Novus' KO cell lysate can be used as a negative control for western blots and to confirm the specificity of antibodies.

Alternate Names for MED15 Knockout HeLa Cell Lysate

• ARC105Arc105
• CAG7A
• CTG repeat protein 7a
• CTG7A
• FLJ42282
• FLJ42935
• mediator complex subunit 15PC2 glutamine/Q-rich-associated protein
• mediator of RNA polymerase II transcription subunit 15
• PC2 (positive cofactor 2, multiprotein complex) glutamine/Q-rich-associatedprotein
• PC2-glutamine-rich-associated protein
• PCQAPDKFZp686A2214
• Positive cofactor 2 glutamine/Q-rich-associated protein
• positive cofactor 2, glutamine/Q-rich-associated protein
• TIG1DKFZp762B1216
• TIG-1TPA-inducible gene 1 protein
• TNRC7
• TPA inducible gene-1
• TPA inducible protein
• trinucleotide repeat containing 7,105-kD
• Trinucleotide repeat-containing gene 7 protein

Background

The protein encoded by this gene is a subunit of the multiprotein complexes PC2 and ARC/DRIP and may function as a transcriptional coactivator in RNA polymerase II transcription. This gene contains stretches of trinucleotide repeats and is located in the chromosome 22 region which is deleted in DiGeorge syndrome. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2014]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Bioinformatics

• Gene Symbol: MED15