Recombinant Human MAPKAP Kinase 3 Protein

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications PAGE
Concentration
0.1 mg/ml

Order Details

Recombinant Human MAPKAP Kinase 3 Protein Summary

Description
Recombinant protein for Human MAPKAP Kinase 3

Source:Baculovirus (Sf9 insect cells)
Amino Acid Sequence:(Q16644)

Source
Sf 9 (baculovirus)
Protein/Peptide Type
Biologically Active Protein
Gene
MAPKAPK3
Purity
>90%, by SDS-PAGE

Applications/Dilutions

Application Notes
Specific activity: 636 nmol phosphate incorporated into MBP protein per minute per mg protein at 30C for 15 minutes using a final concentration of 50 uM ATP (0.83 uCi/assay).
Theoretical MW
69 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -20 to -80C. Avoid freeze-thaw cycles.
Buffer
50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 0.25 mM DTT, 0.1 mM EGTA, 0.1 mM EDTA, 0.1 mM PMSF, 25% glycerol
Concentration
0.1 mg/ml
Purity
>90%, by SDS-PAGE

Alternate Names for Recombinant Human MAPKAP Kinase 3 Protein

  • 3PKEC 2.7.11.1
  • Chromosome 3p kinase
  • EC 2.7.11
  • MAP kinase-activated protein kinase 3
  • MAPK-activated protein kinase 3
  • MAPKAP kinase 3
  • MAPKAP33pK
  • MAPKAPK-3
  • mitogen-activated protein kinase-activated protein kinase 3

Background

MAPKAPK3 has a single potential SH3-binding site in the proline-rich N terminus, a putative ATP-binding site, 2 MAP kinase phosphorylation site motifs, and a putative nuclear localization signal. It shares 72% nucleotide and 75% amino acid identity with MAPKAPK2 (1). MAPKAPK3 was shown to be activated by growth inducers and stress stimulation of cells. In vitro studies demonstrated that ERK, p38 MAP kinase and Jun N-terminal kinase were all able to phosphorylate and activate this kinase, which suggested the role of this kinase as an integrative element of signaling in both mitogen and stress responses (2). This kinase was reported to interact with, phosphorylate and repress the activity of E47, which is a basic helix-loop-helix transcription factor known to be involved in the regulation of tissue-specific gene expression and cell differentiation (3). MAPKAPK3 is uniquely poised to support luteal maturation through the phosphorylation and activation of the nuclear transcription factor CREB (4).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 2 years from date of receipt.

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Bioinformatics

Gene Symbol MAPKAPK3