MAPKAP Kinase 3 Antibody (JU30-31)

Western Blot: MAPKAP Kinase 3 Antibody (JU30-31) [NBP2-75565] - Analysis of MAPKAP Kinase 2 on SK-Br-3 cell lysate using anti-MAPKAP Kinase 2 antibody at 1/500 dilution.

Product Discontinued

MAPKAP Kinase 3 Antibody (JU30-31) Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Synthetic peptide within Human MAPKAP Kinase 3 aa 351-400 / 400. (SwissProt: P49137 Human; SwissProt: P49138 Mouse)
• Localization: Cytoplasm. Nucleus.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: MAPKAPK3
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation 1:10-1:50
  • Western Blot 1:500-1:1000
• Theoretical MW: 46 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: TBS (pH7.4), 0.05% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for MAPKAP Kinase 3 Antibody (JU30-31)

• 3PKEC 2.7.11.1
• Chromosome 3p kinase
• EC 2.7.11
• MAP kinase-activated protein kinase 3
• MAPK-activated protein kinase 3
• MAPKAP kinase 3
• MAPKAP33pK
• MAPKAPK-3
• mitogen-activated protein kinase-activated protein kinase 3

Background

MAPKAP Kinase 3 encodes a member of the Ser/Thr protein kinase family. This kinase functions as a mitogen-activated protein kinase (MAP kinase)- activated protein kinase. MAP kinases are also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals. This kinase was shown to be activated by growth inducers and stress stimulation of cells. In vitro studies demonstrated that ERK, p38 MAP kinase and Jun N-terminal kinase were all able to phosphorylate and activate this kinase, which suggested the role of this kinase as an integrative element of signaling in both mitogen and stress responses. This kinase was reported to interact with, phosphorylate and repress the activity of E47, which is a basic helix-loop-helix transcription factor known to be involved in the regulation of tissue-specific gene expression and cell differentiation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: MAPKAPK3