MAPKAP Kinase 3 Antibody (3F4)

Images

 
Western Blot: MAPKAP Kinase 3 Antibody (3F4) [H00007867-M01] - MAPKAPK3 monoclonal antibody (M01), clone 3F4. Analysis of MAPKAPK3 expression in NIH/3T3.
Western Blot: MAPKAP Kinase 3 Antibody (3F4) [H00007867-M01] - MAPKAPK3 monoclonal antibody (M01), clone 3F4 Analysis of MAPKAPK3 expression in HeLa.
Western Blot: MAPKAP Kinase 3 Antibody (3F4) [H00007867-M01] - Analysis of MAPKAPK3 expression in transfected 293T cell line by MAPKAPK3 monoclonal antibody (M01), clone 3F4.Lane 1: MAPKAPK3 transfected lysate ...read more
Immunoprecipitation: MAPKAP Kinase 3 Antibody (3F4) [H00007867-M01] - Analysis of MAPKAPK3 transfected lysate using anti-MAPKAPK3 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with MAPKAPK3 ...read more
Sandwich ELISA: MAPKAP Kinase 3 Antibody (3F4) [H00007867-M01] - Detection limit for recombinant GST tagged MAPKAPK3 is approximately 0.1ng/ml as a capture antibody.

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, ELISA, IP
Clone
3F4
Clonality
Monoclonal
Host
Mouse

Order Details

MAPKAP Kinase 3 Antibody (3F4) Summary

Immunogen
MAPKAPK3 (AAH01662, 272 a.a. ~ 382 a.a) partial recombinant protein with GST tag.EVSEDAKQLIRLLLKTDPTERLTITQFMNHPWINQSMVVPQTPLHTARVLQEDKDHWDEVKEEMTSALATMRVDYDQVKIKDLKTSNNRLLNKRRKKQAGSSSASQGCNNQ
Specificity
MAPKAPK3 - mitogen-activated protein kinase-activated protein kinase 3
Isotype
IgG2a Kappa
Clonality
Monoclonal
Host
Mouse
Gene
MAPKAPK3
Purity
IgG purified
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Test in a species/application not listed above to receive a full credit towards a future purchase.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Conjugate
Unconjugated
Buffer
PBS (pH 7.4)
Preservative
No Preservative
Purity
IgG purified

Applications/Dilutions

Dilutions
  • Western Blot 1:500
  • ELISA 1:100-1:2000
  • Immunoprecipitation 1:10-1:500
Application Notes
Antibody reactive against cell lysate and recombinant protein for western blot. It has also been used for ELISA.In Simple Western only 10-15 uL of the recommended dilution is used per data point.

Reactivity Notes

Human, Mouse.

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for MAPKAP Kinase 3 Antibody (3F4)

  • 3PKEC 2.7.11.1
  • Chromosome 3p kinase
  • EC 2.7.11
  • MAP kinase-activated protein kinase 3
  • MAPK-activated protein kinase 3
  • MAPKAP kinase 3
  • MAPKAP33pK
  • MAPKAPK-3
  • mitogen-activated protein kinase-activated protein kinase 3

Background

This gene encodes a member of the Ser/Thr protein kinase family. This kinase functions as a mitogen-activated protein kinase (MAP kinase)- activated protein kinase. MAP kinases are also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals. This kinase was shown to be activated by growth inducers and stress stimulation of cells. In vitro studies demonstrated that ERK, p38 MAP kinase and Jun N-terminal kinase were all able to phosphorylate and activate this kinase, which suggested the role of this kinase as an integrative element of signaling in both mitogen and stress responses. This kinase was reported to interact with, phosphorylate and repress the activity of E47, which is a basic helix-loop-helix transcription factor known to be involved in the regulation of tissue-specific gene expression and cell differentiation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Contact Information

Product PDFs

Bioinformatics

Gene Symbol MAPKAPK3
Entrez
OMIM
Uniprot

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