MAPKAP Kinase 3 Antibody (3F4) - Azide and BSA Free

Western Blot: MAPKAP Kinase 3 Antibody (3F4) [H00007867-M01] - MAPKAPK3 monoclonal antibody (M01), clone 3F4. Analysis of MAPKAPK3 expression in NIH/3T3.

Western Blot: MAPKAP Kinase 3 Antibody (3F4) [H00007867-M01] - MAPKAPK3 monoclonal antibody (M01), clone 3F4 Analysis of MAPKAPK3 expression in HeLa.

Western Blot: MAPKAP Kinase 3 Antibody (3F4) [H00007867-M01] - Analysis of MAPKAPK3 expression in transfected 293T cell line by MAPKAPK3 monoclonal antibody (M01), clone 3F4.Lane 1: MAPKAPK3 transfected lysate (Predicted MW: 43 KDa).Lane 2: Non-transfected lysate.

Immunoprecipitation: MAPKAP Kinase 3 Antibody (3F4) [H00007867-M01] - Analysis of MAPKAPK3 transfected lysate using anti-MAPKAPK3 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with MAPKAPK3 monoclonal antibody.

Sandwich ELISA: MAPKAP Kinase 3 Antibody (3F4) [H00007867-M01] - Detection limit for recombinant GST tagged MAPKAPK3 is approximately 0.1ng/ml as a capture antibody.

• Reactivity: Hu, Mu
• Applications: WB, Simple Western, ELISA, IP
• Clone: 3F4
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

MAPKAP Kinase 3 Antibody (3F4) - Azide and BSA Free Summary

• Description: Quality control test: Antibody Reactive Against Recombinant Protein.
• Immunogen: MAPKAPK3 (AAH01662, 272 a.a. ~ 382 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. EVSEDAKQLIRLLLKTDPTERLTITQFMNHPWINQSMVVPQTPLHTARVLQEDKDHWDEVKEEMTSALATMRVDYDQVKIKDLKTSNNRLLNKRRKKQAGSSSASQGCNNQ
• Specificity: MAPKAPK3 - mitogen-activated protein kinase-activated protein kinase 3
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: MAPKAPK3
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunoprecipitation
  • Sandwich ELISA
  • Simple Western 1:50
  • Western Blot
• Application Notes: Antibody reactivity against cell lysate and recombinant protein for WB. It has been used for ELISA.In Simple Western only 10-15 uL of the recommended dilution is used per data point.
  See Simple Western Antibody Database for Simple Western validation: separated by Size, Charge, antibody dilution of 1:50, apparent MW was 50 kDa

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for MAPKAP Kinase 3 Antibody (3F4) - Azide and BSA Free

• 3PKEC 2.7.11.1
• Chromosome 3p kinase
• EC 2.7.11
• MAP kinase-activated protein kinase 3
• MAPK-activated protein kinase 3
• MAPKAP kinase 3
• MAPKAP33pK
• MAPKAPK-3
• mitogen-activated protein kinase-activated protein kinase 3

Background

This gene encodes a member of the Ser/Thr protein kinase family. This kinase functions as a mitogen-activated protein kinase (MAP kinase)- activated protein kinase. MAP kinases are also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals. This kinase was shown to be activated by growth inducers and stress stimulation of cells. In vitro studies demonstrated that ERK, p38 MAP kinase and Jun N-terminal kinase were all able to phosphorylate and activate this kinase, which suggested the role of this kinase as an integrative element of signaling in both mitogen and stress responses. This kinase was reported to interact with, phosphorylate and repress the activity of E47, which is a basic helix-loop-helix transcription factor known to be involved in the regulation of tissue-specific gene expression and cell differentiation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for MAPKAP Kinase 3 Antibody (H00007867-M01)(1)

Publication using H00007867-M01

• Ehlting C, Rex J, Albrecht U et al. Cooperative and distinct functions of MK2 and MK3 in the regulation of the macrophage transcriptional response to lipopolysaccharide. Sci Rep. 2019-07-30 [PMID: 31363109]

Bioinformatics

• Gene Symbol: MAPKAPK3
• Entrez:
  • 7867(Human)
• OMIM:
  • 602130(Human)
• Uniprot:
  • AAH01662(Human)