LPAR1/LPA1/EDG-2 Antibody

Product Discontinued

LPAR1/LPA1/EDG-2 Antibody Summary

• Immunogen: Synthetic peptide, corresponding to amino acids 10-62 of Human EDG-2.
• Clonality: Polyclonal
• Host: Rabbit
• Gene: LPAR1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Immunohistochemistry 1:50-1:200
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Western Blot 1:500-1:1000
• Application Notes: Western blot (WB) analysis of EDG2 (W45) pAb in extracts from Jurkat cells.
  Immunohistochemistry (IHC) analyzes of EDG2 (W45) pAb in paraffin-embedded mouse brain tissue.
• Theoretical MW: 38 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2)
• Preservative: 0.05% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for LPAR1/LPA1/EDG-2 Antibody

• EDG2
• edg-2
• EDG2Lysophosphatidic acid receptor Edg-2
• endothelial differentiation, lysophosphatidic acid G-protein-coupled receptor2,
• GPCR26
• GPR26
• LPA receptor 1
• LPA1
• LPA-1
• LPA1vzg-1
• LPAR1
• lysophosphatidic acid receptor 1
• Mrec1.3
• rec.1.3
• ventricular zone gene 1
• VZG1
• vzg-1

Background

The EDG (endothelial differentiation gene) family of G protein-coupled receptors consists of eight family members that bind lysophospholipid (LPL) mediators, including sphingosine-1-phosphate (SPP) and lysophosphatidic acid (LPA). EDG-1, EDG-3, EDG-5 (also designated H218 and AGR16) and EDG-8 bind SPP with high affinity. EDG-6 is a low affinity receptor for SPP. LPA preferentially binds to EDG2, EDG-4 and EDG-7. The EDG receptors couple to multiple G proteins to signal through Ras, MAP kinase, Rho, Phospholipase C or other tyrosine kinases, which lead to cell survival, growth, migration and differentiation. EDG-1 signals through Gi proteins to activate Akt and is expressed in glioma cells. EDG-2 is expressed in brain, especially in white matter tract regions, while EDG-3 is expressed in cardiovascular tissue and in cerebellum. EDG-4 is highly expressed on leukocytes and brain, and EDG-5 has wide tissue distribution, including cardiovascular tissue and brain. Expressed in lymphoid and hematopoietic tissues and in lung, EDG-6 signals through Gi/o proteins, which activate growth related pathways.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for LPAR1/LPA1/EDG-2 Antibody (NBP1-00788)(2)

Publications using NBP1-00788

• Zhang MZ, Wang X, Yang H et al. Lysophosphatidic Acid Receptor Antagonism Protects against Diabetic Nephropathy in a Type 2 Diabetic Model J Am Soc Nephrol. 2017-07-23 [PMID: 28739650] (IHC-P, WB, Mouse)
• Sokolov E, Eheim AL, Ahrens WA et al. Lysophosphatidic acid receptor expression and function in human hepatocellular carcinoma J Surg Res 2012-11-15 [PMID: 23182454] (WB, Human, Rat)

Bioinformatics

• Gene Symbol: LPAR1
• Entrez:
  • 1902(Human)
  • 116744(Rat)
• Uniprot:
  • Q92633()