LPAR1/LPA1/EDG-2 Antibody (1020714) [Unconjugated]

Human PBMC monocytes were stained with Mouse Anti-Human LPAR1/LPA1/EDG-2 Monoclonal Antibody (Catalog # MAB99631, filled histogram) or Mouse IgG1 Isotype Control Antibody (MAB002, open histogram) followed by anti-Mouse IgG APC-conjugated secondary antibody (F0101B). Staining was performed using our Staining Membrane-associated Proteins protocol.

Product Discontinued

LPAR1/LPA1/EDG-2 Antibody (1020714) [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant human LPAR1/LPA₁/EDG-2
  Met1-Lys50
  Accession # Q92633
• Specificity: Detects human LPAR1/LPA₁/EDG-2 in direct ELISAs.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 0.25 ug/10^6 cells

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for LPAR1/LPA1/EDG-2 Antibody (1020714) [Unconjugated]

• EDG2
• edg-2
• EDG2Lysophosphatidic acid receptor Edg-2
• endothelial differentiation, lysophosphatidic acid G-protein-coupled receptor2,
• GPCR26
• GPR26
• LPA receptor 1
• LPA1
• LPA-1
• LPA1vzg-1
• LPAR1
• lysophosphatidic acid receptor 1
• Mrec1.3
• rec.1.3
• ventricular zone gene 1
• VZG1
• vzg-1

Background

Lysophosphatidic acid receptor 1, LPAR1, also known as EDG-2, is a G protein-coupled receptor that binds the lipid signaling molecule lysophosphatidic acid (LPA). EDG molecules are G protein-coupled receptors that bind plasma lysophospholipids. The EDG family consists of two subfamilies; the S1P (sphingosine-1-phosphate) subfamily consisting of EDG-1, 3, 5, 6, and 8, and the LPA subfamily that contains EDG-2, 4 and 7. The S1P family regulates essential cellular processes such as proliferation, migration, cytoskeletal organization, and differentiation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for LPAR1/LPA1/EDG-2 Antibody (MAB99631)(1)

Publication using MAB99631

• Jong Min Hong, Jin-Woo Lee, Dong-Seung Seen, Jae-Yeon Jeong, Won-Ki Huh LPA1-mediated inhibition of CXCR4 attenuates CXCL12-induced signaling and cell migration Cell Commun Signal 2023-09-25 [PMID: 37749552] (Flow Cytometry, Western Blot, Human)

Bioinformatics

• Uniprot:
  • Q92633()